Curcumin Heals Indomethacin-Induced Gastric Ulceration by Stimulation of Angiogenesis and Restitution of Collagen Fibers via VEGF and MMP-2 Mediated Signaling
EPrints@IICB
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Title |
Curcumin Heals Indomethacin-Induced Gastric Ulceration by Stimulation of Angiogenesis and Restitution of Collagen Fibers via VEGF and MMP-2 Mediated Signaling |
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Creator |
Sharma, Anamika Vivek
Ganguly, Krishnendu Paul, Sumit Maulik, Nilanjana Swarnakar, Snehasikta |
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Subject |
Drug Development/Diagnostics & Biotechnology
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Description |
Aim:We examined the molecular mechanism of curcumin in a preventive and therapeutic model of indomethacininduced gastric ulceration with regard to angiogenic processes. Results: Disrupted blood vessels, reduced collagen matrices, and significant (60%) injury to mucosal cells were observed during ulceration. In addition, ulcerated tissues exhibited decreased matrix metalloproteinase (MMP)-2 and vascular endothelial growth factor (VEGF) expression in blood vessels. Interestingly, curcumin blocked ulceration by induction of collagenization and angiogenesis in gastric tissues via upregulation of MMP-2, membrane type (MT) 1-MMP, VEGF, and transforming growth factor (TGF)-b at protein and messenger ribonucleic acid (mRNA) levels. To examine the angiogenic properties of curcumin, we employed a chorioallantoic membrane model and Matrigel assay. During healing, curcumin promoted collagenization and angiogenesis as well as enhanced MMP-2 activity via positive MT1-MMP regulation and negative tissue inhibitor of metalloproteinase-2 regulation. Innovation: Our study demonstrates that curcumin-mediated healing is associated with increased MMP-2, TGF-b, and VEGF expression and that it plays a pivotal role as an angiogenic modulator by stimulating vascular sprout formation and collagen fiber restoration in ulcerated tissues. Conclusion: We conclude that curcumin remodels gastric tissues by restoring the collagen architecture and accelerating angiogenesis. |
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Date |
2012
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/1480/1/ANTIOXIDANTS_%26__REDOX_SIGNALING_16(_4)351%2D362;2012[61].pdf
Sharma, Anamika Vivek and Ganguly, Krishnendu and Paul, Sumit and Maulik, Nilanjana and Swarnakar, Snehasikta (2012) Curcumin Heals Indomethacin-Induced Gastric Ulceration by Stimulation of Angiogenesis and Restitution of Collagen Fibers via VEGF and MMP-2 Mediated Signaling. Antioxidants & Redox Signaling, 16 (4). pp. 351-362. |
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Relation |
http://dx.doi.org/10.1089/ars.2011.4232
http://www.eprints.iicb.res.in/1480/ |
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