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Curcumin as Anti-Endometriotic Agent: Implication of MMP-3 and Intrinsic Apoptotic Pathway

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Title Curcumin as Anti-Endometriotic Agent: Implication of MMP-3 and Intrinsic Apoptotic Pathway
 
Creator Jana, Sayantan
Paul, Sumit
Swarnakar, Snehasikta
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description The disease of reproductive women, endometriosis represents implantation of functional endometrial
glands outside uterine cavity. This invasive disorder is associated with dysregulation of matrix
metalloproteases (MMP)s and extracellular matrix (ECM) remodeling. In this study, we investigated the
role of MMP-3 on apoptosis during endometriosis. We also checked whether curcumin has potency to
regress endometriosis by modulating MMP-3 and apoptotic pathway. Mouse model of endometriosis
was designed by intraperitoneal inoculation of endometrial tissues to syngeneic female BALB/c. At 15th
day, stable endometriotic developments were observed with increased MMP-3 expression. TUNEL
positive cells were also found with endometriotic progression, which might resulted from destruction of
local immune cells. We speculate that increased MMP-3 activity might be involved in the Fas mediated
apoptosis. Curcumin treatment regressed endometriosis by inhibiting NFkB translocation and MMP-3
expression. It also accelerated apoptosis in endometriomas predominantly via cytochrome-c mediated
mitochondrial pathway. Involvement of mitochondria in apoptosis was further confirmed by atomic
force microscopy (AFM). These results were also supported by our therapeutic study, where curcumin
induced apoptosis both by p53 dependent and independent manner, while celecoxib followed only p53
independent pathway. Altogether, our study establishes the novel role of curcumin as a potent antiendometriotic
compound.
 
Publisher Elsevier
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1492/1/BIOCHEMICAL_PHARMACOLOGY__83(_6)_797%2D804;2012[40].pdf
Jana, Sayantan and Paul, Sumit and Swarnakar, Snehasikta (2012) Curcumin as Anti-Endometriotic Agent: Implication of MMP-3 and Intrinsic Apoptotic Pathway. Biochemical Pharmacology, 83 (6). pp. 797-804.
 
Relation http://dx.doi.org/10.1016/j.bcp.2011.12.030
http://www.eprints.iicb.res.in/1492/