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Nanocapsulated Curcumin: Oral Chemopreventive Formulation against Diethylnitrosamine Induced Hepatocellular Carcinoma in Rat

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Title Nanocapsulated Curcumin: Oral Chemopreventive Formulation
against Diethylnitrosamine Induced Hepatocellular Carcinoma in Rat
 
Creator Ghosh, Debasree
ThakurChoudhury, Somsubhra
Ghosh, Swarupa
Mandal, Ardhendu K
Sarkar, Sibani
Ghosh, Aparajita
Das Saha, Krishna
Das, Nirmalendu
 
Subject Drug Development/Diagnostics & Biotechnology
Infectious Diseases and Immunology
 
Description Toxic outcome of chemical therapeutics as well as multidrug resistance are two serious phenomena for
their inacceptance in cancer chemotherapy. Antioxidants like curcumin (Cur) have gained immense
importance for their excellent anticarcinogenic activities and minimum toxic manifestations in biological
system. However, Cur is lipophilic and thus following oral administration hardly appears in blood indicating
its potential therapeutic challenge in cancer therapy. Nanocapsulated Cur has been used as a drug
delivery vector to focus the effectiveness of these vesicles against hepatocellular carcinoma. The theme of
work was to evaluate effectiveness in oral route of polylactide co-glycolide (PLGA) Nanocapsulated curcumin
(Nano Cur) against diethylnitrosamine (DEN) induced hepatocellular carcinoma (HCC) in rat.
Nano Cur of average diameter 14 nm and encapsulation efficiency of 78% were prepared. Fourier Transform
Infra Red (FTIR) analysis revealed that there is no chemical interaction between drug and the polymer.
Three i.p. injections of the chemical hepatocarcinogen DEN at 15 days interval causes hepatotoxicity,
the generation of reactive oxygen species (ROS), lipid peroxidation, decrease in plasma membrane microviscosity
and depletion of antioxidant enzyme levels in liver. Nano Cur (weekly oral treatment for
16 weeks at 20 mg/kg b.wt) in DEN induced HCC rats exerted significant protection against HCC and
restored redox homeostasis in liver cells. Nanocapsulated Cur caused cancer cell apoptosis as visualized
by ApoBrdU analysis. Histopathological analysis confirmed the pathological improvement in the liver.
Nano Cur was found to be a potential formulation in oral route in combating the oxidative damage of
hepatic cells and eliminating DEN induced hepatocellular cancer cells in rat whereas identical amount
of free Cur treatment was found almost ineffective.
 
Publisher Elsevier
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1504/1/CHEMICO%2DBIOLOGICAL_INTERACTIONS_195(_3)_206%2D214;2012[52].pdf
Ghosh, Debasree and ThakurChoudhury, Somsubhra and Ghosh, Swarupa and Mandal, Ardhendu K and Sarkar, Sibani and Ghosh, Aparajita and Das Saha, Krishna and Das, Nirmalendu (2012) Nanocapsulated Curcumin: Oral Chemopreventive Formulation against Diethylnitrosamine Induced Hepatocellular Carcinoma in Rat. Chemico-Biological Interactions, 195 (3). pp. 206-214.
 
Relation http://dx.doi.org/10.1016/j.cbi.2011.12.004
http://www.eprints.iicb.res.in/1504/