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Intercalation and Induction of Strand Breaks by Adriamycin and Daunomycin: A Study with Human Genomic DNA

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Title Intercalation and Induction of Strand Breaks by Adriamycin
and Daunomycin: A Study with Human Genomic DNA
 
Creator Ghosh, Debjani
Hossain, Maidul
Saha, Chabita
Dey, Subrata Kumar
Kumar, G Suresh
 
Subject Chemistry
 
Description The anticancer drugs Adriamycin (ADR) and Daunomycin (DNM) of the anthracycline family are effective in
treating a variety of cancers. Although their interactions with other cellular targets may play a role in the
selective cytotoxicity of these drugs, it is generally believed that intercalation with DNA is essential for their
activity. However, a relationship has not yet been established between intercalation and cellular processes
leading to cytotoxicity. The present study was designed to investigate the relationship, if any, between intercalation
and DNA strand breaks. ADR and DNM were observed to be strong intercalators of human genomic
DNA by absorption and fluorimetric methods that were further substantiated by rise in thermal melting temperature.
DNM is the better intercalator of the two, which is also evident from circular dichroic spectral changes.
DNA strand breaks, considered to be an index of genotoxicity, was assayed by single cell gel electrophoresis
(SCGE; comet assay). ADR and DNM induced equivalent genotoxicity in normal human lymphocytes at a
clinically used dose, which was observed to be independent of intercalation efficiency though positively correlated
to yield of reactive oxygen species.
 
Publisher Mary Ann Liebert
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1506/1/DNA_AND_CELL_BIOLOGY__31(_3)_377%2D386;2010[45].pdf
Ghosh, Debjani and Hossain, Maidul and Saha, Chabita and Dey, Subrata Kumar and Kumar, G Suresh (2012) Intercalation and Induction of Strand Breaks by Adriamycin and Daunomycin: A Study with Human Genomic DNA. DNA and Cell Biology, 31 (3). pp. 377-386.
 
Relation http://dx.doi.org/10.1089/dna.2011.1299
http://www.eprints.iicb.res.in/1506/