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Radio-Attenuated Leishmanial Parasites as Immunoprophylactic Agent Against Experimental Murine Visceral Leishmaniasis

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Title Radio-Attenuated Leishmanial Parasites as Immunoprophylactic Agent Against Experimental Murine Visceral Leishmaniasis
 
Creator Datta, Sanchita
Adak, Rupchand
Chakraborty, Priyanka
Haldar, Arun Kumar
Bhattacharjee, Surajit
Chakraborty, Anindita
Roy , Syamal
Manna, Madhumita
 
Subject Infectious Diseases and Immunology
 
Description The present study intends to evaluate the role of radio-attenuated leishmania parasites as immunoprophylactic
agents for experimental murine visceral leishmaniasis. BALB/c mice were immunized with
gamma (c)-irradiated Leishmania donovani. A second immunization was given after 15 days of first
immunization. After two immunizations, mice were infected with virulent L. donovani promastigotes.
Protection against Kala-azar (KA) was estimated from spleen and liver parasitic burden along with the
measurement of nitrite and superoxide anion generation by isolation of splenocytes and also by T-lymphocyte
helper 1(Th1) and T-lymphocyte helper 2(Th2) cytokines release from the experimental groups.
It was observed that BALB/c mice having prior immunization with radio-attenuated parasites showed
protection against L. donovani infection through higher expression of Th1 cytokines and suppression of
Th2 cytokines along with the generation of protective free radicals. The group of mice without prior priming
with radio-attenuated parasites surrendered to the disease. Thus it can be concluded that radio-attenuated
L. donovani may be used for.
 
Publisher Elsevier
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1507/1/EXPERIMENTAL_PARASITOLOGY__130(_1)_39%2D47;20121[77].pdf
Datta, Sanchita and Adak, Rupchand and Chakraborty, Priyanka and Haldar, Arun Kumar and Bhattacharjee, Surajit and Chakraborty, Anindita and Roy , Syamal and Manna, Madhumita (2012) Radio-Attenuated Leishmanial Parasites as Immunoprophylactic Agent Against Experimental Murine Visceral Leishmaniasis. Experimental Parasitology, 130 (1). pp. 39-47.
 
Relation http://dx.doi.org/10.1016/j.exppara.2011.10.001
http://www.eprints.iicb.res.in/1507/