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Interleukin-8 Gene Regulation in Epithelial Cells by Vibrio Cholerae: Role of Multiple Promoter Elements, Adherence and Motility of Bacteria and Host MAPKs

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Title Interleukin-8 Gene Regulation in Epithelial Cells by
Vibrio Cholerae: Role of Multiple Promoter Elements,
Adherence and Motility of Bacteria and Host MAPKs
 
Creator Sarkar, Madhubanti
Bhowmick, Swati
Casola, Antonella
Chaudhuri, Keya
 
Subject Molecular & Human Genetics
 
Description Interleukin (IL)-8 is an important mediator in neutrophil-mediated acute
inflammation. We previously demonstrated that incubation of intestinal
epithelial cells with Vibrio cholerae O395 resulted in increased IL-8 mRNA
expression and IL-8 secretion, which was associated with the adherence
and motility of bacteria. However, the mechanisms responsible for transcriptional
regulation of the IL-8 gene in epithelial cells during V. cholerae
infections were not explored. Transient transfection analysis of 5¢ deletions
and mutations of the IL-8 promoter driving expression of the luciferase
reporter gene indicates that multiple binding sites contribute to IL-8 promoter
induction in response to V. cholerae and that cooperation among
these different sites is required for full activation of the promoter. Stimulation
with V. cholerae O395 insertional mutants, defective in adherence and
motility, significantly reduced IL-8 promoter activity compared with the
wild-type strain. We further demonstrate maximal involvement of extracellular
signal-regulated kinase 1 ⁄ 2 ⁄ mitogen-activated protein kinase pathways
to regulate IL-8 gene transcription. This study will help to design agents
which could reduce the V. cholerae-induced inflammatory response and in
the generation of safe vaccines.
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1508/1/FEBS_JOURNAL__279_(_8)1464%2D1473;2012[29].pdf
Sarkar, Madhubanti and Bhowmick, Swati and Casola, Antonella and Chaudhuri, Keya (2012) Interleukin-8 Gene Regulation in Epithelial Cells by Vibrio Cholerae: Role of Multiple Promoter Elements, Adherence and Motility of Bacteria and Host MAPKs. FEBS Journal, 279 (8). pp. 1464-1473.
 
Relation http://dx.doi.org/10.1111/j.1742-4658.2012.08539.x
http://www.eprints.iicb.res.in/1508/