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Recognition of Different DNA Sequences by a DNA-Binding Protein Alters Protein Dynamics Differentially

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Title Recognition of Different DNA Sequences by a DNA-Binding Protein Alters Protein Dynamics Differentially
 
Creator Mondol, Tanumoy
Batabyal, Subrata
Mazumder, Abhishek
Roy, Siddhartha
 
Subject Structural Biology & Bioinformatics
 
Description k-Repressor–operator sites interaction, particularly OR1 and OR2, is a key component of the k-genetic
switch. FRET from the dansyl bound to the C-terminal domain of the protein, to the intercalated EtBr
in the operator DNA indicates that the structure of the protein is more compact in the OR2 complex
than in the OR1 complex. Fluorescence anisotropy reveals enhanced flexibility of the C-terminal
domain of the repressor at fast timescales after complex formation with OR1. In contrast, OR2 bound
repressor shows no significant enhancement of protein dynamics at these timescales. These differences
are shown to be important for correct protein–protein interactions. Altered protein dynamics
upon specific DNA sequence recognition may play important roles in assembly of regulatory proteins
at the correct positions.
 
Publisher Elsevier
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1509/1/FEBS_LETTERS__586_(_3_)_258%2D262;2012[53].pdf
Mondol, Tanumoy and Batabyal, Subrata and Mazumder, Abhishek and Roy, Siddhartha (2012) Recognition of Different DNA Sequences by a DNA-Binding Protein Alters Protein Dynamics Differentially. FEBS Letters, 586 (3). pp. 258-262.
 
Relation http://dx.doi.org/10.1016/j.febslet.2011.12.032
http://www.eprints.iicb.res.in/1509/