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Induction of Cr(VI) Reduction Activity in an Anoxybacillus Strain under Heat Stress: a Biochemical and Proteomic Study

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Title Induction of Cr(VI) Reduction Activity in an Anoxybacillus Strain under Heat Stress: a Biochemical and Proteomic Study
 
Creator Chowdhury Paul, Sangita
Jain, Preeti
Mitra, Jyotirmoy
Dutta, Sangita
Bhattacharya, Pamela
Bal, Bijay
Bhattacharyya, Debasish
Das Gupta, Sujoy
Pal, Subrata
 
Subject Structural Biology & Bioinformatics
 
Description A bacterial strain, designated as TSB-6, was isolated from the sediments of a
Tantloi (India) hot spring at 65 °C. The strain showed 98% 16S rRNA gene
sequence similarity with Anoxybacillus kualawohkensis strain KW12 and was
found to grow optimally at 37 °C. However, growing cells, cell suspensions,
and cell-free extracts from 65 °C cultures showed higher Cr(VI) reduction
activities when assayed at either 37 or 65 °C than those obtained from 37 °C
cultures. On fractionation of extracts from cells grown at 65 °C, the chromate
reductase activity assayed at 65 °C was found mostly in the soluble fraction.
When log-phase cells growing at 37 °C were shifted to 65 °C, the stressed cells
produced larger quantities of reactive oxygen species. Consequently, growth of
the cells was retarded, but specific Cr(VI) reduction activity increased. 2D gel
electrophoresis followed by MALDI-TOF MS/MS identified the proteins whose
expression level changed as a result of heat stress. The upregulated set included
proteins involved in cellular metabolism of sugar, nucleotide, amino acids,
lipids and vitamins, oxidoreductase activity, and protein folding. The downregulated
proteins are also involved in cellular metabolism, DNA binding, and
environmental signal processing.
 
Publisher Elsevier
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1510/1/FEMS_MICROBIOLOGY_LETTERS__331(_1)_70%2D80;2012[8].pdf
Chowdhury Paul, Sangita and Jain, Preeti and Mitra, Jyotirmoy and Dutta, Sangita and Bhattacharya, Pamela and Bal, Bijay and Bhattacharyya, Debasish and Das Gupta, Sujoy and Pal, Subrata (2012) Induction of Cr(VI) Reduction Activity in an Anoxybacillus Strain under Heat Stress: a Biochemical and Proteomic Study. FEMS Microbiology Letters, 331 (1). pp. 70-80.
 
Relation http://dx.doi.org/10.1111/j.1574-6968.2012.02555.x
http://www.eprints.iicb.res.in/1510/