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Identification and Molecular Characterization of an Alba-Family Protein from Human Malaria Parasite Plasmodium Falciparum

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Title Identification and Molecular Characterization of an
Alba-Family Protein from Human Malaria Parasite
Plasmodium Falciparum
 
Creator Goyal, Manish
Alam, Athar
Iqbal, Mohd Shameel
Dey, Sumanta
Bindu, Samik
Pal, Chinmay
Banerjee, Anindyajit
Chakrabarti, Saikat
Bandyopadhyay, Uday
 
Subject Infectious Diseases and Immunology
Structural Biology & Bioinformatics
 
Description We have investigated the DNA-binding nature as
well as the function of a putative Alba (Acetylation
lowers binding affinity) family protein (PfAlba3)
from Plasmodium falciparum. PfAlba3 possesses
DNA-binding property like Alba family proteins.
PfAlba3 binds to DNA sequence non-specifically
at the minor groove and acetylation lowers its
DNA-binding affinity. The protein is ubiquitously
expressed in all the erythrocytic stages of P. falciparum
and it exists predominantly in the acetylated
form. PfAlba3 inhibits transcription in vitro by
binding to DNA. Plasmodium falciparum Sir2
(PfSir2A), a nuclear localized deacetylase interacts
with PfAlba3 and deacetylates the lysine residue of
N-terminal peptide of PfAlba3 specific for DNA
binding. PfAlba3 is localized with PfSir2A in the periphery
of the nucleus. Fluorescence in situ hybridization
studies revealed the presence of PfAlba3 in
the telomeric and subtelomeric regions. ChIP and
ChIP ReChIP analyses further confirmed that
PfAlba3 binds to the telomeric and subtelomeric
regions as well as to var gene promoter.
 
Publisher Oxford University Press
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1532/1/NUCLEIC_ACIDS_RESEARCH___40_(_3_)_1174%2D1190;2012[57].pdf
Goyal, Manish and Alam, Athar and Iqbal, Mohd Shameel and Dey, Sumanta and Bindu, Samik and Pal, Chinmay and Banerjee, Anindyajit and Chakrabarti, Saikat and Bandyopadhyay, Uday (2012) Identification and Molecular Characterization of an Alba-Family Protein from Human Malaria Parasite Plasmodium Falciparum. Nucleic Acids Research, 40 (3). pp. 1174-1190.
 
Relation http://dx.doi.org/10.1093/nar/gkr821
http://www.eprints.iicb.res.in/1532/