Record Details

Structural Diversity in Bacterial Ribosomes: Mycobacterial 70S Ribosome Structure Reveals Novel Features

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Structural Diversity in Bacterial Ribosomes:
Mycobacterial 70S Ribosome Structure Reveals Novel
Features
 
Creator Shasmal, Manidip
Sengupta, Jayati
 
Subject Structural Biology & Bioinformatics
 
Description Here we present analysis of a 3D cryo-EM map of the 70S ribosome from Mycobacterium smegmatis, a saprophytic cousin of
the etiological agent of tuberculosis in humans, Mycobacterium tuberculosis. In comparison with the 3D structures of other
prokaryotic ribosomes, the density map of the M. smegmatis 70S ribosome reveals unique structural features and their
relative orientations in the ribosome. Dramatic changes in the periphery due to additional rRNA segments and extra
domains of some of the peripheral ribosomal proteins like S3, S5, S16, L17, L25, are evident. One of the most notable
features appears in the large subunit near L1 stalk as a long helical structure next to helix 54 of the 23S rRNA. The sharp
upper end of this structure is located in the vicinity of the mRNA exit channel. Although the M. smegmatis 70S ribosome
possesses conserved core structure of bacterial ribosome, the new structural features, unveiled in this study, demonstrates
diversity in the 3D architecture of bacterial ribosomes. We postulate that the prominent helical structure related to the 23S
rRNA actively participates in the mechanisms of translation in mycobacteria
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1539/1/PLOS_ONE__7(_2_)_Article_Number_e31797;2012[48].pdf
Shasmal, Manidip and Sengupta, Jayati (2012) Structural Diversity in Bacterial Ribosomes: Mycobacterial 70S Ribosome Structure Reveals Novel Features. PLoS ONE, 7 (7). e31742.
 
Relation http://dx.doi.org/10.1371/journal.pone.0031742
http://www.eprints.iicb.res.in/1539/