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Chemotypical variations in Withania Somnifera Lead to Differentially Modulated Immune Response in BALB/c Mice

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Title Chemotypical variations in Withania Somnifera Lead to Differentially Modulated Immune Response in BALB/c Mice
 
Creator Kushwaha, Susheela
Roy, Saptarshi
Maity, Rita
Mallick, Asish
Sonia, Vishal K
Singha, Prashant K
Chaurasiya, Narayan D
Sangwan, Rajender S
Misra-Bhattacharya, Shailja
Mandal, Chitra
 
Subject Infectious Diseases and Immunology
 
Description Withania somnifera (Ashwagandha) is a plant with known ethnomedicinal properties and its use in
Ayurvedic medicine in India is well documented. The present investigation reports on immunomodulatory
efficacy of aqueous-ethanol extracts of roots of three selected Withania somnifera chemotypes
designated as NMITLI 101R, NMITLI 118R and NMITLI 128R. Each chemotype was administered
10–100 mg/kg orally to BALB/c mice once daily for 14 days. The immunomodulatory consequences were
recorded by determining the humoral immune response with the help of hemagglutination, plaque forming
cell assay and cellular response by measuring delayed type hypersensitivity reaction. Additionally,
other immune parameters such as proliferation of T and B cells, intracellular and secreted Th1 and Th2
cytokines along with modulation in ROS production by peritoneal macrophages were monitored after
feeding with lower doses (3–30 mg/kg/day) of these three chemotypes individually. NMITLI 101R incited
both humoral and cellular immune response in terms of higher number of antibody producing cells and
enhanced foot pad swelling at the 10 mg dose as also dose dependent B and T cell proliferations. Levels
of intracellular and secreted cytokines post-NMITLI 101R treatment illustrated generation of mixed
Th1/Th2 response that remained more polarized towards Th1. This chemotype also generated maximum
reactive oxygen species. NMITLI 118R provoked comparatively reduced immune response in all humoral
and cellular parameters at lower doses but induced highly polarized Th1 cytokine response. In contrast,
NMITLI 128R led to enhanced antibody production with minimal cellular response demonstrating
marginally Th2 dominance at a lower dose. Taken together, it may therefore be concluded that there were
distinct modulation in the immune response exhibited by the three chemotypes of Withania somnifera
and NMITLI 101R appeared to possess a better immunostimulatory activity than the other chemotypes
at lower doses.
 
Publisher Elsevier
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1551/1/VACCINE_30_(6)_1083%2D1093;2012[55].pdf
Kushwaha, Susheela and Roy, Saptarshi and Maity, Rita and Mallick, Asish and Sonia, Vishal K and Singha, Prashant K and Chaurasiya, Narayan D and Sangwan, Rajender S and Misra-Bhattacharya, Shailja and Mandal, Chitra (2012) Chemotypical variations in Withania Somnifera Lead to Differentially Modulated Immune Response in BALB/c Mice. Vaccine, 30 (6). pp. 1083-1093.
 
Relation http://dx.doi.org/10.1016/j.vaccine.2011.12.031
http://www.eprints.iicb.res.in/1551/