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Mustard Stalk and Straw: A New Source for Production of Lignocellulolytic Enzymes by the Fungus Termitomyces Clypeatus and as a Substrate for Saccharification

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Title Mustard Stalk and Straw: A New Source for Production of Lignocellulolytic
Enzymes by the Fungus Termitomyces Clypeatus and as a Substrate for Saccharification
 
Creator Pal, Swagata
Banik, Samudra Prosad
Khowala, Suman
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Agro residue of mustard obtained as mustard stalk and straw (MSS) was investigated for the first time
for production of lignocellulolytic enzymes by Termitomyces clypeatus and also for use as substrate for
saccharification. MSS with high cellulose and hemicellulose content was utilized as sole source of carbon
by the fungus for productions of enzymes such as (CMcase, �-glucosidase, xylanase and �-xylosidase) in
submerged fermentation. Production of enzymes were further increased by 2–10 folds on supplementation
with common agro-residues such as wheat bran and rice straw (MWR) in 1:1:1 ratio and by using
alkali treated MSS (TMSS). The enzymes obtained from MWR and TMSS media could saccharify 10% (w/v)
wheat bran up to 53% and 58% in 24 h, and xylan up to 52% and 81% in 12 h, respectively. MSS was used
for saccharification by enzymes of T. clypeatus grown in cellulose media after pretreatment with hot
water and NaCl respectively, where extent of saccharification was doubled to 80% by salt treatment as
compared to that with hot water. The results indicated that MSS can be used as a potential and cheap
renewable raw material from India for production of bio-ethanol
 
Publisher Elsevier
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1608/1/INDUSTRIAL_CROPS_AND_PRODUCTS___(41)_283%2D288;2012[100].pdf
Pal, Swagata and Banik, Samudra Prosad and Khowala, Suman (2012) Mustard Stalk and Straw: A New Source for Production of Lignocellulolytic Enzymes by the Fungus Termitomyces Clypeatus and as a Substrate for Saccharification. Industrial Crops and Products, 41. pp. 283-288.
 
Relation http://dx.doi.org/10.1016/j.indcrop.2012.04.022
http://www.eprints.iicb.res.in/1608/