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IL-4 Alone without the Involvement of GM-CSF Transforms Human Peripheral Blood Monocytes to a CD1adim, CD83+ Myeloid Dendritic Cell Subset

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Title IL-4 Alone without the Involvement of GM-CSF Transforms Human Peripheral Blood Monocytes to a CD1adim, CD83+ Myeloid Dendritic Cell Subset
 
Creator Roy, Keshab Chandra
Bandyopadhyay, Gautam
Rakshit, Srabanti
Ray, Mitali
Bandyopadhyay, Santu
 
Subject Infectious Diseases and Immunology
 
Description Myeloid dendritic cells (DCs) are conventionally generated
by culturing human peripheral blood monocytes in the
presence of GM-CSF and IL-4. Here we report that IL-4
alone, in the absence of detectable endogenous GM-CSF,
transforms human peripheral blood monocytes to a
CD1adim DC subset that could be matured to CD83+
DCs. Absence of endogenous GM-CSF in IL-4-DC was
demonstrated by RT-PCR and flow cytometry. With the
exception of CD1a expression, surface marker, morphology
and phagocytic activity of these DCs (IL-4-DC) were
similar to myeloid DCs (GM-IL-4-DC) conventionally
generated in the presence of GM-CSF and IL-4.
Conventional GM-IL-4-DC produced less IL-12 compared with IL-4-DC after stimulation with anti-CD40 monoclonal
antibody, or LPS plus IFN-g, although the difference was
more prominent when LPS plus IFN-g was used as the
stimulus. The GM-IL-4-DC also induced less frequent IFN-
g+ T cells in a mixed leukocyte reaction (MLR) than that of
IL-4-DC. Yields of IL-4-DCs were marginally lower than
that of GM-IL-4-DCs. Our data indicate that peripheral
blood monocytes can be transformed to CD1a-deficient
myeloid DCs solely by IL-4, and these IL-4-DCs are likely
to induce a stronger Th1 response than conventional GMIL-
4-DCs
 
Publisher Company of Biologists
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1626/1/JCS%2C_2004.pdf
Roy, Keshab Chandra and Bandyopadhyay, Gautam and Rakshit, Srabanti and Ray, Mitali and Bandyopadhyay, Santu (2004) IL-4 Alone without the Involvement of GM-CSF Transforms Human Peripheral Blood Monocytes to a CD1adim, CD83+ Myeloid Dendritic Cell Subset. Journal of Cell Science (JCS), 117 (16). pp. 3435-3445.
 
Relation http://dx.doi.org/10.1242/​jcs.01162
http://www.eprints.iicb.res.in/1626/