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Leishmania donovani Infection of Human Myeloid Dendritic Cells Leads to a Th1 Response in CD4+ T Cells from Healthy Donors and Patients with Kala-Azar.

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Title Leishmania donovani Infection of Human Myeloid Dendritic Cells Leads to a Th1 Response in CD4+ T Cells from Healthy Donors and Patients with Kala-Azar.
 
Creator Ghosh, Monidipa
Mandal, Labanya
Maitra, Sudipta
Rakshit, Srabanti
Paul, Kausik
Bagchi, Jayashree
Ganguly, Dipyaman
Pal, Chiranjib
Bandyopadhyay, Santu
 
Subject Infectious Diseases and Immunology
 
Description The role played by dendritic cells (DCs) in Leishmania donovani infection is poorly understood. Here, we
report that L. donovani amastigotes efficiently infect human peripheral-blood monocyte–derived DCs. Opsonization
with normal human serum enhanced the infectivity of amastigotes and promastigotes only marginally.
Surface attachment versus internalization was distinguished by incubation of DCs with live, fluorescein isothiocyanate–labeled parasites, followed by quenching with crystal violet. Infection with amastigotes was accompanied by DC maturation, as was evident from the up-regulation of maturation-associated cell-surface markers, the nuclear translocation of RelB, and the release of cytokines. Amastigote-primed DCs produced inflammatory cytokines in response to subsequent treatment with interferon-g or anti-CD40 monoclonal antibody. When cocultured, amastigote-infected DCs induced T helper cell type 1 (Th1) responses both in naive allogeneic CD4+ T cells and in autologous CD4+ T cells from patients with kala-azar and up-regulated the expression of T-bet. Our data reveal that infection with L. donovani amastigotes induces a Th1 cytokine milieu in both DCs and T cells.
 
Publisher University of Chicago Press
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1627/1/JID%2C_2006.pdf
Ghosh, Monidipa and Mandal, Labanya and Maitra, Sudipta and Rakshit, Srabanti and Paul, Kausik and Bagchi, Jayashree and Ganguly, Dipyaman and Pal, Chiranjib and Bandyopadhyay, Santu (2006) Leishmania donovani Infection of Human Myeloid Dendritic Cells Leads to a Th1 Response in CD4+ T Cells from Healthy Donors and Patients with Kala-Azar. The Journal of Infectious Diseases, 194. pp. 294-301.
 
Relation http://dx.doi.org/
http://www.eprints.iicb.res.in/1627/