Record Details

Visceral Leishmaniasis in India: Promises and Pitfalls of a PCR-Based Blood Test

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Visceral Leishmaniasis in India: Promises and Pitfalls of a PCR-Based Blood Test
 
Creator Adhya, Samit
Hassan, Md Quamarul
Mukherjee, Sandeep
Manna, Partha Pratim
Basu, Anirban
Sen, Sandeep
Bandyopadhyay, Santu
 
Subject Infectious Diseases and Immunology
Molecular & Human Genetics
 
Description Traditional methods of diagnosing visceral leishmaniasis (kala-azar) in India suffer from a number of
disadvantages. Amplification of multicopy nuclear genes and messenger ribonucleic acid of Leishmania
by the polymerase chain reaction (PCR) was evaluated as an alternative assay under various clinical
conditions. PCR of peripheral blood has the highest absolute sensitivity among all the available
procedures, and is particularly useful for detecting parasites in early infections, post kala-azar dermal
leishmaniasis, concurrent infections and immunocompromised cases, but is not so reliable for late
infections. PCR of immunopurified blood mononuclear cells indicated the association of parasites with
monocytes as well as non-monocyte cell types.
 
Publisher Elsevier
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1640/1/Trans._Royal_Society_of_Trop._Med._and_Hygiene%2C_2002.pdf
Adhya, Samit and Hassan, Md Quamarul and Mukherjee, Sandeep and Manna, Partha Pratim and Basu, Anirban and Sen, Sandeep and Bandyopadhyay, Santu (2002) Visceral Leishmaniasis in India: Promises and Pitfalls of a PCR-Based Blood Test. Transactions of The Royal Society of Tropical Medicine and Hygiene, 96. S1/179-S1/183.
 
Relation http://dx.doi.org/
http://www.eprints.iicb.res.in/1640/