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Inhibition of toxic actions of phospholipase A2 isolated & characterized from the Indian Banded Krait (Bungarus fasciatus) venom by synthetic herbal compounds

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Title Inhibition of toxic actions of phospholipase A2 isolated & characterized from the Indian Banded Krait (Bungarus fasciatus) venom by synthetic herbal compounds
 
Creator Gomes, Antony
Bhattacharya, Shamik
Mukherjee,, Sanghamitra
Tsai, Inn-ho-
Gomes, Aparna Gomes*
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Phospholipase A2 (PLA2) is one of the major constituents of krait venom
associated with several pathophysiological actions like myotoxicity, cardiotoxicity, neurotoxicity, 12&6
As there was no specific antiserum available against $+,(%)+57%5&3%2+5 venom, this study was done
with synthetic herbal compounds, anti PLA2 rabbit antiserum and commercial polyvalent snake venom
antiserum to neutralize the PLA2 induced toxicities in experimental models.
venom phospholipase A2 fraction 38 (BF-38) was isolated by ion exchange
chromatography, molecular weight was determined by mass spectrometry and its N terminal amino
acid sequence was identified. Monospecific rabbit antiserum was raised against the PLA2 in presence of
Freund complete adjuvant. The neutralization of PLA2 induced toxicities was done in 3,432)*and in 434* 3, models using synthetic herbal compounds, anti PLA2 rabbit antiserum and commercial polyvalent
snake venom antiserum.
A toxic PLA2 (BF-38) was purified from the $:7%5&3%2+5 venom by CM-cellulose and HPLC, of 13.17
kDa and a minor band of 7.3 kDa using ESI-MS. The 13.17 kDa PLA2 sequence was NLYQFKNMIQC.
The 7.3 kDa toxin sequence was RKCLTKYSQDNES and was found to be
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1680/1/IJMR_paper.pdf
Gomes, Antony and Bhattacharya, Shamik and Mukherjee,, Sanghamitra and Tsai, Inn-ho- and Gomes, Aparna Gomes* (2012) Inhibition of toxic actions of phospholipase A2 isolated & characterized from the Indian Banded Krait (Bungarus fasciatus) venom by synthetic herbal compounds. Indian Journal of Medical Research, 136. pp. 40-45.
 
Relation http://dx.doi.org/
http://www.eprints.iicb.res.in/1680/