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Cytotoxic and Antioxidant Property of a Purified Fraction (NN-32) of Indian Naja Naja Venom on Ehrlich Ascites Carcinoma in BALB/c Mice

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Title Cytotoxic and Antioxidant Property of a Purified Fraction (NN-32) of
Indian Naja Naja Venom on Ehrlich Ascites Carcinoma in BALB/c Mice
 
Creator Das, Tanaya
Bhattacharya, Shamik
Halder, Babli
Biswas, Archita
Das Gupta, Shubho
Gomes, Antony
Gomes, Aparna
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description A cytotoxic and antioxidant protein (NN-32) from the Indian spectacled cobra Naja naja
venom was identified and its probable mode of action on murine Ehrlich ascites carcinoma
(EAC) was established. The venom purified through ion exchange chromatography
produced several peaks, among which fraction 32 produced cytotoxic-cardiotoxic properties.
This fraction (NN-32) showed a single peak (retention time 38.3 min) by HPLC using
C4 column. The molecular mass determined by MALDI-MS, found to be 6.7 kDa and the
first ten N-terminal sequence was determined (LKCNKLVPLF) by Edmann degradation
method using applied Biosystem procise sequencer. It was observed that the sequence
shared 100% homology with other cytotoxin cardiotoxin identified from the venom of Naja
species. NN-32 showed cytotoxicity on EAC cells, increased survival time of inoculated EAC
mice, reduced solid tumor volume and weight. NN-32 increased proapoptotic protein
caspase 3 and 9 activity and Bax-Bcl2 ratio. It also increased the antioxidant markers
glutathione, glutathione peroxidase, glutathione transferase, superoxide dismutase and
catalase activity. NN-32 increased serum IL-10 level and decreased murine keratinocytederived
chemokine level. The cardiotoxicity of NN-32 was established on isolated guinea
pig auricle, where 100% irreversible blockade of auricular contraction was observed. Thus,
it may be concluded that, NN-32 induced anticancer activity in EAC mice was partly
mediated through its apoptogenic – antioxidant property.
 
Publisher Elsevier
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1681/1/new_paper_of_us@Toxicon.pdf
Das, Tanaya and Bhattacharya, Shamik and Halder, Babli and Biswas, Archita and Das Gupta, Shubho and Gomes, Antony and Gomes, Aparna (2011) Cytotoxic and Antioxidant Property of a Purified Fraction (NN-32) of Indian Naja Naja Venom on Ehrlich Ascites Carcinoma in BALB/c Mice. Toxicon, 57. pp. 1065-1072.
 
Relation http://dx.doi.org/10.1016/j.toxicon.2011.04.012
http://www.eprints.iicb.res.in/1681/