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Shedding off Specific Lipid Constituents from Sperm Cell Membrane during Cryopreservation

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Title Shedding off Specific Lipid Constituents from Sperm Cell Membrane during Cryopreservation
 
Creator Chakrabarty, Jitamanyu
Banerjee, Dipankar
Pal, Debasish
De, Joydeep
Ghosh, Amitabha
Majumder, Gopal Chandra
 
Subject Cell Biology & Physiology
 
Description Membrane damage is one of the main reasons for reduced motility and fertility of sperm cells during cryopreservation. Using a model
system of sperm cryopreservation developed in our laboratory, we have investigated the detailed changes due to cryopreservation in the
plasma membrane lipid composition of the goat epididymal sperm cells. Total lipid and its components, i.e., neutral lipids, glycolipids
and phospholipids decreased significantly after cryopreservation. Among neutral lipids sterols, steryl esters and 1-O-alkyl-2,3-diacyl glycerols
decreased appreciably, while among phospholipids, major loss was observed for phosphatidyl choline and phosphatidyl ethanolamine.
Unsaturated fatty acids bound to the phospholipids diminished while the percentage of saturated acids increased. The
cholesterol:phospholipid ratio enhanced and the amount of hydrocarbon, which was unusually high, increased further on cryopreservation.
The data indicates that profound increase of the hydrophobicity of the cell membrane is one of the major mechanisms by which
spermatozoa acquire potential to resist or combat stress factors like cryodamage. The results are compatible with the view that for survival
against cryodamage, sperm cells modulate the structure of their outer membrane by shedding off preferentially some hydrophilic
lipid constituents of the cell membrane.
 
Publisher Elsevier
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1702/1/CRYOBIOLOGY___54_(_1)_27%2D35;2007[115].pdf
Chakrabarty, Jitamanyu and Banerjee, Dipankar and Pal, Debasish and De, Joydeep and Ghosh, Amitabha and Majumder, Gopal Chandra (2007) Shedding off Specific Lipid Constituents from Sperm Cell Membrane during Cryopreservation. Cryobiology, 54. pp. 27-35.
 
Relation http://dx.doi.org/10.1016/j.cryobiol.2006.10.191
http://www.eprints.iicb.res.in/1702/