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Human intestinal epithelial cell cytokine mRNA responses mediated by NF-�B are modulated by the motility and adhesion process of Vibrio cholerae

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Title Human intestinal epithelial cell cytokine mRNA responses mediated by NF-�B are modulated by the motility and adhesion process of Vibrio cholerae
 
Creator Bandyopadhaya, Arunava
Sarkar, Madhubanti
Chaudhuri, Keya
 
Subject Molecular & Human Genetics
 
Description Vibro cholerae, the etiological agent of cholera, colonizes the small intestine, produces an enterotoxin and causes acute inflammatory
response at intestinal epithelial surface; the signals for such induction are still unknown.We determined the mRNA expression
of proinflammatory and anti-inflammatory cytokines in Int407 cells following infection with V. cholerae or its mutants by semiquantitaive
and quantitative real-time RT-PCR. V. cholerae induces the coordinated expression and up-regulation of IL-1�, IL-6,
GM-CSF and MCP-1 and down-regulation of TGF-� in Int407 cells. While the pathogenecity of V. cholerae was found to be
a possible determinant in modulation of IL-1� and TGF-�, both IL-6 and MCP-1 OmpU might modulate induction. Significant
reduction in IL-1�, GM-CSF and MCP-1 mRNA expression was observed upon infection with the less motile and less adherent
strain O395YN. This association is supported by the absence of nuclear translocation of NF-�B (p50 subunit) upon infection with
O395YN in contrast to wild-type. Moreover, TPCK treatment prior to V. cholerae infection indicated that proinflammatory cytokine
gene expression in Int407 cells is NF-�B mediated. Thus, V. cholerae induces proinflammatory cytokine response in Int407 cells,
which is mediated by NF-�B and is modulated, in part, by adherence or motility of this organism.
 
Publisher Elsevier
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1708/1/INTERNATIONAL_JOURNAL_OF_BIOCHEMISTRY_%26_CELL_BIOLOGY___39(_10)_1863%2D1876;2007[131].pdf
Bandyopadhaya, Arunava and Sarkar, Madhubanti and Chaudhuri, Keya (2007) Human intestinal epithelial cell cytokine mRNA responses mediated by NF-�B are modulated by the motility and adhesion process of Vibrio cholerae. The International Journal of Biochemistry & Cell Biology, 39 (10). pp. 1863-1876.
 
Relation http://dx.doi.org/10.1016/j.cryobiol.2006.10.191
http://www.eprints.iicb.res.in/1708/