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Deglycosylation Effect of the Mammalian Sperm Maturation Antigen (SMA2) on Serological Reaction and Acrosome Reaction

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Title Deglycosylation Effect of the Mammalian Sperm Maturation Antigen
(SMA2) on Serological Reaction and Acrosome Reaction
 
Creator Das, Tapasi
Chatterjee, Tapati
 
Subject Infectious Diseases and Immunology
 
Description Spermatozoal membrane proteins are considered to possess several immunological unique characteristics as the cell is formed behind the blood-testes barriers. Major goat sperm maturation antigen (SMA2) contains one hexosamine along with mannose, galactose and glucose. In the present study, effects of deglycosylation of SMA2 antigen on immunoreactivity and the serological activity was investigated. SMA2 glycoantigen showed positive
immunoreactivity after treatment with sodium borohydride (NaBH4) and moreover this generated a 44 kDa protein band which was negative for periodic acid Schiff reagent. Trifluoromethanesulfonic acid (TFMS) caused aggregation and restricted the free mobility of the treated antigen on SDS-PAGE and the protein band generated by TFMS treatment also showed positive immuno-reactivity. The results supported the views that the protein portion retains its immuno-reactivity even after oxidation of the vicinal hydroxyl group of saccharide component of SMA2 antigen. These data suggest that immunodominent epitopes exist on the core protein by which the SMA2 antigen retains its immuno-reactivity
even after disruption of the saccharide portion. Additional experiments demonstrate that protein epitopes have a role in capacitation and the acrosome reaction (AR) in presence of antibody which is raised against this protein part of SMA2 using the negative staining of FITC-PSA (fluorescein isothiocyanate-labeled Pisum sativum agglutinin) probe. Altogether these findings indicate that the protein portion of SMA2 might fulfill the serological activity of the antigen as well as the protein epitope affects the acrosome reaction. In view of this property, we propose that the protein portion of SMA2 antigen might be considered as a potential antigenic target for an immune response.
 
Publisher Elsevier
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1712/1/ANIMAL_REPRODUCTION_SCIENCE___133(_3%2D4)176%2D183;2012[56].pdf
Das, Tapasi and Chatterjee, Tapati (2012) Deglycosylation Effect of the Mammalian Sperm Maturation Antigen (SMA2) on Serological Reaction and Acrosome Reaction. Animal Reproduction Science, 133 (3-4). pp. 176-183.
 
Relation http://dx.doi.org/10.1016/j.anireprosci.2012.07.001
http://www.eprints.iicb.res.in/1712/