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Induced Biofilm Cultivation Enhances Riboflavin Production by an Intertidally Derived Candida Famata

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Title Induced Biofilm Cultivation Enhances Riboflavin Production by an Intertidally Derived Candida Famata
 
Creator Mitra, Sayani
Thawrani, Dheeraj
Banerjee, Priyam
Gachhui, Ratan
Mukherjee, Joydeep
 
Subject Cell Biology & Physiology
 
Description The aim of the investigation was to ascertain if surface attachment of Candida
famata and aeration enhanced riboflavin production. A newly designed polymethylmethacrylate
(PMMA) conico-cylindrical flask (CCF) holding eight equidistantly spaced rectangular
strips mounted radially on a circular disk allowed comparison of riboflavin production between
CCFs with hydrophobic surface (PMMA-CCF), hydrophilic glass surface (GS-CCF), and 500-
ml Erlenmeyer flask (EF). Riboflavin production (mg/l) increased from 12.79 to 289.96, from
54.44 to 238.14, and from 36.98 to 158.71 in the GS-CCF, EF, and PMMA-CCF, respectively,
when C. famata was grown as biofilm-induced cultures in contrast to traditional planktonic
culture. Production was correlated with biofilm formation and planktonic growth was suppressed
in cultivations that allowed higher biofilm formation. Enhanced aeration increased
riboflavin production in hydrophilic vessels. Temporal pattern of biofilm progression based on
two-channel fluorescence detection of extracellular polymeric substances and whole cells in a
confocal laser scanning microscope followed by application of PHLIP and ImageJ volume
viewer software demonstrated early maturity of a well-developed, stable biofilm on glass in
contrast to PMMA surface. A strong correlation between hydrophilic reactor surface, aeration,
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1723/1/APPLIED_BIOCHEMISTRY_AND_BIOTECHNOLOGY__166_(8_)1991%2D2006;2012[127].pdf
Mitra, Sayani and Thawrani, Dheeraj and Banerjee, Priyam and Gachhui, Ratan and Mukherjee, Joydeep (2012) Induced Biofilm Cultivation Enhances Riboflavin Production by an Intertidally Derived Candida Famata. Applied Biochemistry and Biotechnology, 166 (8). pp. 1991-2006.
 
Relation http://dx.doi.org/:10.1007/s12010-012-9626-7
http://www.eprints.iicb.res.in/1723/