Record Details

Sandwich Immunoassay of Small Molecules II. Effects of Heterology and Development of a Highly Sensitive and Specific Enzyme Immunoassay for Testosterone

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Sandwich Immunoassay of Small Molecules
II. Effects of Heterology and Development of a Highly Sensitive and Specific
Enzyme Immunoassay for Testosterone
 
Creator Sengupta, Judhajit
Dhar, Tarun K
Ali, Esahak
 
Subject Infectious Diseases and Immunology
 
Description The effect of dimer heterology in the sandwich immunoassay of testosterone was studied using
symmetrical and asymmetrical dimers prepared from testosterone-3-(O-carboxymethyl)oxime and 4-
(carboxymethyl-mercapto)testosterone. The effect of antibody heterology was studied using antibodies
against 3-carboxymethyl oxime and 17-hemisuccinate derivatives of the steroid and an asymmetrical
dimer prepared from the same two derivatives. Using antibody against the 3-carboxymethyl oxime and
the dimer of 4-(carboxymethylmercapto)testosterone, the sensitivity of direct enzyme immunoassay of
testosterone in serum by this method was 0.5 pg/well and the cross-reactivity of 5a-dihydrotestosterone
was 5%.
 
Publisher Elsevier
 
Date 1992
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1793/1/JOURNAL_OF_IMMUNOLOGICAL_METHODS__147_(_2)_181%2D188_;1992[50].pdf
Sengupta, Judhajit and Dhar, Tarun K and Ali, Esahak (1992) Sandwich Immunoassay of Small Molecules II. Effects of Heterology and Development of a Highly Sensitive and Specific Enzyme Immunoassay for Testosterone. Journal of Immunological Methods, 142 (2). pp. 181-188.
 
Relation http://dx.doi.org/
http://www.eprints.iicb.res.in/1793/