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Binding of Plant Alkaloids Berberine and Palmatine to Serum Albumins: A Thermodynamic Investigation

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Title Binding of Plant Alkaloids Berberine and Palmatine to Serum
Albumins: A Thermodynamic Investigation
 
Creator Khan, Asma Yasmeen
Hossain, Maidul
Kumar, G Suresh
 
Subject Chemistry
 
Description The thermodynamics of the interaction of two
pharmaceutically important isoquinoline alkaloids berberine
and palmatine with bovine and human serum albumin was
investigated using calorimetric techniques, and the data was
supplemented with fluorescence and circular dichroism
studies. Thermodynamic results revealed that there was only
one class of binding sites for both alkaloids on BSA and
HSA. The equilibrium constant was of the order of 104 M-1
for both the alkaloids to serum albumins but the magnitude
was slightly higher with HSA. Berberine showed higher
affinity over palmatine to both proteins. The binding was
enthalpy dominated and entropy favoured for both the alkaloids
to BSA and HSA. Salt dependent studies suggested that
electrostatic interaction had a significant role in the binding
process, the binding affinity reduced as the salt concentration
increased. Temperature dependent calorimetric data yielded
heat capacity values that suggested the involvement of different
molecular forces in the complexation of the two alkaloids
with BSA and HSA. 3D fluorescence, synchronous
fluorescence and circular dichroism data suggested that the
binding of the alkaloids changed the conformation of proteins
by reducing their helicity. Destabilization of the protein
conformation was also revealed from differential scanning
calorimetry studies. Overall, the alkaloids bound strongly to
serum albumins, but berberine was a better binder to both
serum proteins compared to palmatine
 
Publisher Kluwer
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1816/1/MOLECULAR_BIOLOGY_REPORTS___40(_1)553%2D566;2013[22].pdf
Khan, Asma Yasmeen and Hossain, Maidul and Kumar, G Suresh (2013) Binding of Plant Alkaloids Berberine and Palmatine to Serum Albumins: A Thermodynamic Investigation. Molecular Biology Reports, 40 (1). pp. 553-566.
 
Relation http://dx.doi.org/10.1007/s11033-012-2092-z
http://www.eprints.iicb.res.in/1816/