Record Details

Organization and Chromosomal Localization of ß-Tubulin Genes in Leishmania Donovani

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Organization and Chromosomal Localization of ß-Tubulin Genes in
Leishmania Donovani
 
Creator Das, Saumitra
Adhya, Samit
 
Subject Molecular & Human Genetics
 
Description The genomic organization and chromosomal location of the ß-tubulin isogenes
in Leishmania donovani promastigotes has been studied by nucleic acid hybridization techniques using a cloned ß-tubulin gene. We have cloned a ß-tubulin gene fragment, 3·3 kbp long, from genomic DNA of Leishmania donovani using a heterologous ß-tubulin DNA as probe. Restriction maps of this clone have been prepared. It has been estimated that there are approximately 11-15 copies of the ß-tubulin genes per haploid genome. The majority of these isogenes are arranged in a tandem repeat with a length of 3·5 kbp on a single chromosome. In addition a few dispersed gene copies at different chromosomal loci were detected by pulse field gradient gel electrophoresis. Part of the internal coding region of the gene has been sequenced to confirm the identity of the ß-tubulin clone and is found to be nearly identical to that of Leishmania mexicana amazonensis.
 
Publisher Cambridge University Press
 
Date 1990
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1825/1/JOURNAL_OF_BIOSCIENCES___15_(_4)_239%2D248;1990[3].pdf
Das, Saumitra and Adhya, Samit (1990) Organization and Chromosomal Localization of ß-Tubulin Genes in Leishmania Donovani. Journal of BioSciences, 15 (4). pp. 239-248.
 
Relation http://dx.doi.org/10.1007/BF02702665
http://www.eprints.iicb.res.in/1825/