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IL-15 activated human peripheral blood dendritic cell kill allogeneic and xenogeneic endothelial cells via apoptosis

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Title IL-15 activated human peripheral blood dendritic cell kill allogeneic and xenogeneic endothelial cells via apoptosis
 
Creator Manna, Partha Pratim
Hira, Sumit Kumar
Das, Apabrita Ayan
Bandyopadhyay, Santu
Gupta, Kailash Kumar
 
Subject Infectious Diseases and Immunology
 
Description IL-15 is a pleotropic cytokine, which plays an important role in natural killer (NK) cell activity, T cell proliferation,
and T cell cytotoxic activity. Dendritic cells (DCs) are the major antigen presenting cells in the
immune system and presumed to play an important role in immune recognition of allo and xenotransplantation.
We showed that IL-15 activated human peripheral blood DC is cytotoxic to human and porcine
aortic endothelial cells. Unlike DCs, CD14+ monocytes show no cytotoxicity against the endothelial
cells. This cytotoxic potential of IL-15 activated DC against endothelial cells is dose dependent and
increases significantly upon treatment of endothelial cells with inflammatory cytokines like TNF-a or
IFN-c. The cytotoxic potential of IL-15 activated DC is associated with apoptosis of endothelial cells, as
indicated by the increased Annexin V staining, caspase activation and loss of mitochondrial membrane
potential. Further it was observed that DC mediated cytotoxicity against endothelial cell is mediated
via granzyme B possibly secreted by the activated DCs.
 
Publisher Elsevier
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1862/1/CYTOKINE___61(_1)_118%2D126;2013[27].pdf
Manna, Partha Pratim and Hira, Sumit Kumar and Das, Apabrita Ayan and Bandyopadhyay, Santu and Gupta, Kailash Kumar (2013) IL-15 activated human peripheral blood dendritic cell kill allogeneic and xenogeneic endothelial cells via apoptosis. Cytokine, 61 (1). pp. 118-126.
 
Relation http://dx.doi.org/10.1016/j.cyto.2012.09.004
http://www.eprints.iicb.res.in/1862/