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Evaluation of Spermicidal Activity of Mi-Saponin A

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Title Evaluation of Spermicidal Activity of Mi-Saponin A
 
Creator Saha, P
Majumda, S
Pal, D
Pal, Bikas C
Kabir, S N
 
Subject Cell Biology & Physiology
 
Description The seed extracts of Madhuca latifolia were reported to have spermicidal activity. The current investigation
identified the spermicidal component of the extracts and evaluated its spermicidal potential in vitro.
As characterized by infrared, mass, and nuclear magnetic resonance (NMR) spectral analyses,Mi-saponin A (MSA) was found to be the most potent component among a mixture of saponins. The mean effective concentrations of MSA that induced irreversible immobilization were 320 mg/mL for rat
and 500 mg/mL for human sperm, as against the respective concentrations of 350 and 550 mg/mL of nonoxynol
9 (N-9). The mode of spermicidal action was evaluated by a battery of tests including (a) double fluoroprobe
staining for spermviability, (b) hypoosmotic swelling test and, assays for 5’ nucleotidase and acrosin for physiological integrity of sperm plasmamembrane, (c) scanning and transmission electron microscopy for sperm
membrane ultrastructure, and (d) plasma membrane lipid peroxidation (LPO). The observations, taken
together,were interpreted to mean that the spermicidal effect ofMSAinvolved increased membraneLPOleading
to structural and functional disintegration of spermplasmamembrane and acrosomal vesicle.Acomparative
in vitro cytotoxicity study in human vaginal keratocyte (Vk2/E6E7) and endocervical (End/E6E7) cell lines
demonstrated that the 50% cell cytotoxicity (CC50) values, and consequently the safety indices, for MSA were� 8-fold higher as compared to those ofN-9. In conclusion,MSA is a potent spermicidal molecule that may be explored further for its suitability as an effective component of vaginal contraceptive.
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1886/1/454.full.pdf
Saha, P and Majumda, S and Pal, D and Pal, Bikas C and Kabir, S N (2010) Evaluation of Spermicidal Activity of Mi-Saponin A. Reproductive Sciences, 17 (5). pp. 454-464.
 
Relation http://www.eprints.iicb.res.in/1886/