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Introduction of Bacterial Components in Postadsorbed Plasma During Adsorption With Staphylococcus aureus

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Title Introduction of Bacterial Components in Postadsorbed Plasma
During Adsorption With Staphylococcus aureus
 
Creator Bandyopadhyay, Santu
Ray, Prasanta K
 
Subject Infectious Diseases and Immunology
 
Description In ritro plasma adsorption over either protein A-positive Staphylococcus aureus Cowan I (SAC) or
protein A-negative S. aureus Wood 46 (SAW) led to leaching of bacterial biomolecules in the
postadsorbed plasma. Presence of bacterial moieties was demonstrated in the postadsorbed plasma by
more than one method: (1) using radiolabeled bacteria for adsorption with plasma and detecting
radioactivity in the postadsorbed plasma, (2) gel filtration of pre- and post-adsorbed plasmas over
Sephadex C-200 column and detecting additional peak(s) in the postadsorbed plasma, and (3)
immunoelectrophoretic analysis of pre- and postadsorbed plasmas and their column fractions against
rabbit anti-SAC antisera and demonstrating new precipitin bands in postadsorbed plasmas. Using an
extracorporeal plasma adsorption procedure in mongrel dogs, with radiolabeled SAC as the adsorbent,
we have demonstrated the presence of radioactivity in both the adsorbed and filtered (0.2 wm) blood
entering into the body, and the adsorbed blood that passed out of the body to reenter into the
extracorporeal circuit. These data suggest that components of S. aureus origin enter into the host
circulation during both in virro and ex vivo plasma adsorption, although the exact nature of those
extracted staphylococcal components remains unknown. This observation is of much significance since
it can possibly help elucidate the mechanism of tumor regression observed following perfusion of
plasma over SAC or SAW, followed by its reinfusion to the host.
 
Date 1985
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1900/1/Cancer%2C56_266%2D272;_1985.pdf
Bandyopadhyay, Santu and Ray, Prasanta K (1985) Introduction of Bacterial Components in Postadsorbed Plasma During Adsorption With Staphylococcus aureus. Cancer, 56. pp. 266-272.
 
Relation http://www.eprints.iicb.res.in/1900/