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Requirement for hla-dr + accessory cells in natural killing of cytomegalovirus-infected fibroblasts

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Title Requirement for hla-dr + accessory cells in natural killing of cytomegalovirus-infected fibroblasts
 
Creator Bandyopadhyay, Santu
Perussia, Bice
Trinchieri, Giorgio
Miller, Deborah S
Starr, Stuart E.
 
Subject Infectious Diseases and Immunology
 
Description NK cells mediate spontaneous killing of tumor-derived cells, virus-infected cells, and certain normal cells (1, 2). This type of cytotoxicity does not require
presensitization of the donor. For example, PBMC of individuals who are seronegative or seropositive for a given virus are equally able to lyse targets
infected with that virus (3). Production of IFN by lymphocytes exposed to virusinfected target cells and subsequent stimulation of NK cells by IFN was originally
proposed as the mechanism by which NK cells preferentially lyse virus-infected cells compared with uninfected ones (4). A primary role for IFN was challenged, however, by several authors who described a lack of correlation between magnitude of lysis and amounts of IFN detected in supernatant fluids (5, 6), an almost normal capacity of effector cells from patients with reduced ability to
produce IFN to lyse virus-infected target cells (7), and the inability of anti-IFN antibodies when present during the NK assay to prevent lysis of virus-infected
cells (5, 6). The cells responsible for NK activity against normal and tumor-derived target cell lines were identified as a leukocyte subset, distinct from B and T cells and from myelomonocytic cells (2). This subset expresses the low-affinity Fc receptor (FcR) 1 for aggregated IgG (CD16 antigen), recognized by a series of mAbs (8). NK cells responsible for lysis of virus-infected target cells have not been fully identified. Fitzgerald et al. (9) reported that the NK cells able to lyse HSVinfected targets differed from those that lysed K562 cells, as treatment of PBMC
with an mAb to HLA-DR plus C reduced their ability to kill HSV-infected fibroblasts, but not K562 cells. These authors concluded that these NK cell subsets could be distinguished on the basis of surface expression of HLA-DR
antigen. However, few if any resting NK (CD16+) cells in healthy donors are HLA-DR + (10, 1 1), raising the possibility that in the experiments of Fitzgerald
et a]. (9), a HLA-DR +, non-NK cell population was depleted.
 
Publisher Rockefeller University Press
 
Date 1986
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1907/1/J._Exp._Medicine%2C_%2C164__180%2D195;1986.pdf
Bandyopadhyay, Santu and Perussia, Bice and Trinchieri, Giorgio and Miller, Deborah S and Starr, Stuart E. (1986) Requirement for hla-dr + accessory cells in natural killing of cytomegalovirus-infected fibroblasts. The Journal of Experimental Medicine (JEM), 164. pp. 180-195.
 
Relation http://www.eprints.iicb.res.in/1907/