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Development Of Inhibitors Of DNA Topoisomerase(s) Of Leishmania Donovani With Potent Antileishmanial Activities And Study On The Mechanism Of Drug Resistance

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Title Development Of Inhibitors Of DNA Topoisomerase(s) Of Leishmania Donovani With Potent Antileishmanial Activities And Study On The Mechanism Of Drug Resistance
 
Creator Chowdhury, Sayan
 
Subject Infectious Diseases and Immunology
 
Description It is a unicellular, protozoan parasite, which exists as motile promastigotes in the sandfly gut and is transmitted into the human host where it transforms into sessile amastigotes that manifests a dreadful clinical symptom called Leishmaniasis. It was discovered at the turn
of the 19th century, viz. by Cunningham, Leishman, Donovan, Borovsky, Wright and Vianna (Gardener et al., 1977). But the name Leishmania donovani was given by Ross in 1903.
Form and Structure In the sandfly midgut, Leishmania exists as an elongated, flagellated, motile promastigote
form. These are usually 12 m (Figure 1) and sometimes as short as 5 m in size. The kinetoplast is anterior in position and free flagellum measures upto 15 m in length. Very small, unattached promastigotes found in the proboscis of sandflies are believed to be the infective form introduced into the mammals (Sacks and Perkins, 1984).
Upon entry into host macrophage, it internalizes in the macrophage and transforms into round shaped, nonflagellated,
non-motile amastigotes in 12-24 hrs (Bachinger, 1987; Alexander and Vickermann, 1975). Amastigotes are spherical in shape (Figure 2), only about 2.5-5 m in diameter and are contained within a parasitophagus vacuole within a macrophage. There is a prominent nucleus and kinetoplast,
the cytoplasm is vacuolated and contains lysosomes. The outer membrane has a polysaccharide component but there is no surface coat. Amastigotes of different species of Leishmania in mammalian hosts are reported to be
morphologically identical. Although the general configuration of amastigote is similar, size has been shown to vary between species (Scorza et al.,1979). Figure 2 shows different forms of Leishmania forms present in sand-fly gut and mammalian host. Metacyclic promastigotes
are the infective stage of Leishmania parasites.
 
Date 2013
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1915/1/Sayan_Chowdhury_HKM_2013_CU.pdf
Chowdhury, Sayan (2013) Development Of Inhibitors Of DNA Topoisomerase(s) Of Leishmania Donovani With Potent Antileishmanial Activities And Study On The Mechanism Of Drug Resistance. PhD thesis, Calcutta University.
 
Relation http://www.eprints.iicb.res.in/1915/