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Phenazinium dyes methylene violet 3RAX and indoine blue bind to DNA by intercalation: Evidence from structural and thermodynamic studies

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Title Phenazinium dyes methylene violet 3RAX and indoine blue bind to DNA by intercalation: Evidence from structural and thermodynamic studies
 
Creator Saha, Ishita
Kumar, G Suresh
 
Subject Chemistry
 
Description The structural and energetic aspects of the interaction of two important phenazinium dyes methylene
violet 3RAX and indoine blue with DNA were studied by spectroscopic and thermochemical techniques.
The binding affinity values of both the dyes were of the order of 105 M�1 but methylene violet 3RAX
bound to DNA more strongly. Both dyes bound DNA by intercalation but methylene violet 3RAX bound
stronger than indoine blue. The DNA binding of both the dyes was exothermic and favoured by a small
negative enthalpy and positive entropy contributions. The effect of ionic strength indicated that
electrostatic attraction is an important component of the dyeeDNA interaction although the major
contribution comes from hydrophobic forces. The temperature dependence of enthalpy changes yielded
negative heat capacity value which was higher for methylene violet 3RAX compared to indoine
blue suggesting higher contribution from hydrophobic forces in the interaction of the former.
Enthalpyeentropy compensation phenomenon was observed for the binding of both dyes to DNA.
 
Publisher Elsevier
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1962/1/DYES_AND_PIGMENTS__Volume_96__(_1)_81%2D91;2013[151].pdf
Saha, Ishita and Kumar, G Suresh (2013) Phenazinium dyes methylene violet 3RAX and indoine blue bind to DNA by intercalation: Evidence from structural and thermodynamic studies. Dyes and Pigments, 96 (1). 81-91`.
 
Relation http://dx.doi.org/10.1016/j.dyepig.2012.07.026
http://www.eprints.iicb.res.in/1962/