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Successful Therapy of Visceral Leishmaniasis With Curdlan Involves T-Helper 17 Cytokines

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Title Successful Therapy of Visceral Leishmaniasis With Curdlan Involves T-Helper 17 Cytokines
 
Creator Ghosh, Kuntal
Sharma, Gunjan
Saha, Amrita
Kar, Susanta
Das, Pijush K
Ukil, Anindita
 
Subject Infectious Diseases and Immunology
 
Description The aim of this study was to evaluate and characterize the therapeutic potential of curdlan, a naturally occurring
β-glucan immunomodulator, against visceral leishmaniasis, a fatal parasitic disease. Curdlan eliminated
the liver and spleen parasite burden in a 45-day BALB/c mouse model of visceral leishmaniasis at a dosage of
10 mg/kg/day as determined by Giemsa-stained organ impression smears. Curdlan was associated with production
of the disease-resolving T-helper (Th) 1 and Th17-inducing cytokines interleukin (IL)-6, IL-1β, and
IL-23, as well as with production of Th17 cytokines IL-17 and IL-22, as determined by enzyme-linked immunosorbent
assay (ELISA) and real time polymerase chain reaction (RT-PCR). Reversal of curdlan-mediated
protection by anti-IL-17 and anti-IL-23 monoclonal antibodies showed the importance of Th17 cytokines.
Significantly decreased production of both IL-17 and IL-22 by mice that received anti-IL-23 antibody
suggested the essential role of IL-23 in Th17 differentiation. Although administration of recombinant IL-17
or IL-23 caused significant suppression of the organ parasite burden, with marked generation of interferon γ
and nitric oxide (NO), effects were much faster for IL-17. These results documented that although both IL-23
and IL-17 play major roles in the antileishmanial effect of curdlan, the effect of IL-23 may occur indirectly,
through the induction of IL-17 production
 
Publisher University of Chicago Press
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/1981/1/JOURNAL_OF_INFECTIOUS_DISEASES__207_(_6)_1016%2D1025;2013[53].pdf
Ghosh, Kuntal and Sharma, Gunjan and Saha, Amrita and Kar, Susanta and Das, Pijush K and Ukil, Anindita (2013) Successful Therapy of Visceral Leishmaniasis With Curdlan Involves T-Helper 17 Cytokines. The Journal of Infectious Diseases, 207 (6). pp. 1016-1025.
 
Relation http://dx.doi.org/10.1093/infdis/jis771
http://www.eprints.iicb.res.in/1981/