Structural characterization of Type III Secretion System Structural characterization of Type III Secretion Systemrelated translocator and effector from Pseudomonas aeruginosa. .
EPrints@IICB
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Title |
Structural characterization of Type III Secretion System Structural characterization of Type III Secretion Systemrelated translocator and effector from Pseudomonas aeruginosa. . |
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Creator |
Dey, Supratim
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Subject |
Structural Biology & Bioinformatics
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Description |
Pseudomonas aeruginosa, a Gram-negative pathogen utilizes a specialized set of Type III Secretion System (T3SS) translocator and effector proteins to establish virulence in the host cell. An understanding of these pathogenic factors that play a key role in the establishment and maintenance of bacterial pathogenicity are thus, of immense importance, and are likely the interest of study of several research groups. The T3SS encoding “translocator operon” of P. aeruginosa consists of a major translocator protein PopB, minor translocator protein PopD and their cognate chaperone Pcpresent a comprehensive study of PopB structure, its interaction with PcrH and their associated pH-based structural and functional changes. The pH-dependent studies indicate that PcrH not only provides structural support to the ordered molten globule PopB in complex but also undergoes conformational change to assist PopB to pass through.SPR studies determine the terminal domains of PopB to be involved in hydrophobic interaction with PcrH as well as maintain the oligomerisation of translocator complex |
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Date |
2013
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Type |
Thesis
NonPeerReviewed |
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Format |
application/pdf
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Identifier |
http://www.eprints.iicb.res.in/1999/1/SupratimThesis_Final.pdf
Dey, Supratim (2013) Structural characterization of Type III Secretion System Structural characterization of Type III Secretion Systemrelated translocator and effector from Pseudomonas aeruginosa. . PhD thesis, Calcutta University . |
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Relation |
http://www.eprints.iicb.res.in/1999/
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