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Proteomic profiling of γ-ECS overexpressed transgenic Nicotiana in response to drought stress

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Title Proteomic profiling of γ-ECS overexpressed transgenic
Nicotiana in response to drought stress
 
Creator Kumar, Deepak
Datta, Riddhi
Sinha, Ragini
Ghosh, Aparupa
Chattopadhyay, Sharmila
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description The contribution of Glutathione (GSH ) in drought stress tolerance is an established fact. However, the proteins
which are directly or indirectly related to the increased level of GSH in response to drought stress are yet to be
known. To explore this, here, transgenic tobacco plants (NtGp11) overexpressing gamma-glutamylcysteine synthetase
(γ-ECS) was tested for tolerance against drought stress. NtGp11 conferred tolerance to drought stress by increased
germination rate, water retention, water recovery, chlorophyll, and proline content compared with wild-type plants.
Semi-quantitative RT-PCR analysis revealed that the transcript levels of stress-responsive genes were higher in
NtGp11 compared with wild-type in response to drought stress. Two-dimensional gel electrophoresis (2-DE) coupled
with MALDI TOF-TOF MS/MS analysis has been used to identify 43 differentially expressed proteins in response to
drought in wild-type and NtGp11 plants. The results demonstrated the up-accumulation of 58.1% of proteins among
which 36%, 24%, and 20% of them were related to stress and defense, carbon metabolism and energy metabolism
categories, respectively. Taken together, our results demonstrated that GSH plays an important role in combating
drought stress in plants by inducing stress related genes and proteins like HSP70, chalcone synthase, glutathione
peroxidase, thioredoxin peroxidase, ACC oxidase, and heme oxygenase I.
 
Publisher Landes Bioscience
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2079/1/2015.pdf
Kumar, Deepak and Datta, Riddhi and Sinha, Ragini and Ghosh, Aparupa and Chattopadhyay, Sharmila (2014) Proteomic profiling of γ-ECS overexpressed transgenic Nicotiana in response to drought stress. Plant Signaling & Behavio, 9. ISSN 1559-2316
 
Relation http://dx.doi.org/10.4161/psb.29246
http://www.eprints.iicb.res.in/2079/