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Investtiigattiion on riibosome structure and dynamiics:: Mycobactteriium riibosome iin focus

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Title Investtiigattiion on riibosome structure and dynamiics:: Mycobactteriium riibosome iin focus
 
Creator Shasmal, Manidip
 
Subject Structural Biology & Bioinformatics
 
Description This doctoral study deals primarily with the application of cryo-EM and single particle reconstruction technique to investigate three major aspects of translation machinery in prokaryotes. Kinetic studies indicate that in vivo, protein synthesis and ribosome biogenesis involves interaction of additional factors, capable of enhancing efficient assembly and protein synthesis. The first part of the study deals with the characterization of hitherto unknown association of cellular protein factors with the bacterial ribosome and functional importance of such interactions with the ribosome. Biochemical studies and a 3D cryo-EM map of a ribosome sample purified directly from cell extract revealed strong association of two cellular proteins namely Aldehyde alcohol dehydrogenase (AdhE) and Outer membrane protein C (OmpC) with E. coli 70S ribosome. Additional ribosome associated functions of these two proteins reflect multi-tasking activity of proteins within cell. The second part of the study deals with structural characterization of ribosome from bacteria that are of clinical importance to human health like Mycobacterium. The 12Å resolution 3D cryo-EM map of Mycobacterium smegmatis revealed unique additional structural components in the mycobacterial ribosome. Identification of several rRNA helices particularly a rRNA helix lying close to mRNA exit and extensions of ribosomal proteins around the peptide exit tunnel in M. smegmatis 70S ribosome structure reflects diversity of ribosome structure across bacterial kingdom and involvement of species specific structural elements in fine tuning the process of protein biosynthesis. The third part of the study addresses structure of the largest ribosomal protein S1 and its interaction with ribosomes from E. coli and M. smegmatis. Dynamic ribosome binding nature of S1 protein in a species specific manner has been explored. The study also presents a model of full length S1 protein from E. coli and interprets the organization of the domains and interaction of S1 with mRNA as well as ribosomal components.
 
Date 2015
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2092/1/MANIDIP_SHASMAL_THESIS.pdf
Shasmal, Manidip (2015) Investtiigattiion on riibosome structure and dynamiics:: Mycobactteriium riibosome iin focus. PhD thesis, Calcutta University.
 
Relation http://www.eprints.iicb.res.in/2092/