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Ellagic Acid, a Dietary Polyphenol, Inhibits Tautomerase Activity of Human Macrophage Migration Inhibitory Factor and Its Pro-inflammatory Responses in Human Peripheral Blood Mononuclear Cells

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Title Ellagic Acid, a Dietary Polyphenol, Inhibits Tautomerase Activity of Human Macrophage Migration Inhibitory Factor and Its Pro-inflammatory Responses in Human Peripheral Blood Mononuclear Cells
 
Creator Sarkar, Souvik
Siddiqui, Asim A
Mazumder, Somnath
De, Rudranil
Saha, Shubhra J.
Banerjee, Chinmoy
Iqbal, Mohd. S
Adhikari, Susanta
Alam, Athar
Roy, Siddhartha
Bandyopadhyay, Uday
 
Subject Infectious Diseases and Immunology
Structural Biology & Bioinformatics
 
Description Ellagic acid (EA), a phenolic lactone, inhibited tautomerase activity of human macrophage migration inhibitory factor (MIF) noncompetitively (Ki = 1.97 ± 0.7 μM). The binding of EA to MIF was determined by following the quenching of tryptophan fluorescence. We synthesized several EA derivatives, and their structure−activity relationship studies indicated that the planar conjugated lactone moiety of EA was essential for MIF inhibition. MIF induces nuclear translocation of NF-κB and chemotaxis of peripheral blood mononuclear cells (PBMCs) to promote inflammation. We were interested in evaluating the
effect of EA on nuclear translocation of NF-κB and chemotactic activity in human PBMCs in the presence of MIF. The results showed that EA inhibited MIF-induced NF-κB nuclear translocation in PBMCs, as evident from confocal immunofluorescence microscopic data. EA also inhibited MIF-mediated chemotaxis of PBMCs. Thus, we report MIF-inhibitory activity of EA and inhibition of MIF-mediated proinflammatory responses in PBMCs by EA.
 
Publisher American Chemical Society
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2220/1/JOURNAL_OF_AGRICULTURAL_AND_FOOD_CHEMISTRY__V._63__(_20_)_4988%2D4998_;2015[13].pdf
Sarkar, Souvik and Siddiqui, Asim A and Mazumder, Somnath and De, Rudranil and Saha, Shubhra J. and Banerjee, Chinmoy and Iqbal, Mohd. S and Adhikari, Susanta and Alam, Athar and Roy, Siddhartha and Bandyopadhyay, Uday (2015) Ellagic Acid, a Dietary Polyphenol, Inhibits Tautomerase Activity of Human Macrophage Migration Inhibitory Factor and Its Pro-inflammatory Responses in Human Peripheral Blood Mononuclear Cells. Journal of Agricultural and Food Chemistry, 63 (20). pp. 4988-4998.
 
Relation http://dx.doi.org/10.1021/acs.jafc.5b00921
http://www.eprints.iicb.res.in/2220/