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Role of the Flagellar Hook-Length Control Protein FliK and σ28 in cagA Expression in Gastric Cell–Adhered Helicobacter pylori

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Title Role of the Flagellar Hook-Length Control Protein FliK and σ28 in cagA Expression in Gastric Cell–Adhered Helicobacter pylori
 
Creator Baidya, Amit K.
Bhattacharya, Saurabh
Chowdhury, Rukhsana
 
Subject Infectious Diseases and Immunology
 
Description Adherence of Helicobacter pylori to the gastric epithelial cell line AGS strongly induces expression of fliK encoding
a flagellar hook-length control protein. FliK has a role in triggering dissociation of the alternate sigma actor, σ28, from a nonfunctional σ28-FlgM complex, releasing free, functional σ28. The σ28-RNA polymerase nitiates transcription of cagA, the major virulence gene, from a promoter identified in this study. Consequently,
significant up-regulation of cagA was observed in AGS-adhered H. pylori. Direct binding of σ28 to the cagA
promoter was demonstrated by chromatin immunoprecipitation and the transcription start site was identified y 5′ RACE (rapid amplification of complementary DNA ends). The σ28-dependent cagA promoter was active pecifically in AGS-adhered H. pylori, and this motif might be associated with high cagA expression and severity f disease. These results also indicate that H. pylori has evolved to integrate expression of the major virulence ene cagA with the flagellar regulatory circuit, essential for colonization of the human host.
 
Publisher University of Chicago Press
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2230/1/JOURNAL_OF_INFECTIOUS_DISEASES__V._211__(_11_)_1779%2D1789_;2015[8].pdf
Baidya, Amit K. and Bhattacharya, Saurabh and Chowdhury, Rukhsana (2015) Role of the Flagellar Hook-Length Control Protein FliK and σ28 in cagA Expression in Gastric Cell–Adhered Helicobacter pylori. The Journal of Infectious Diseases, 211 (11). pp. 1779-1789. (In Press)
 
Relation http:/dx.doi.org/10.1093/infdis/jiu808
http://www.eprints.iicb.res.in/2230/