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Excited State Proton Transfer in the Lysosome of Live Lung Cells:Normal and Cancer Cells

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Title Excited State Proton Transfer in the Lysosome of Live Lung Cells:Normal and Cancer Cells
 
Creator Chowdhury, Rajdeep
Saha, Abhijit
Mandal, Amit Kumar
Jana, Batakrishna
Ghosh, Surajit
Bhattacharyya, Kankan Bhattacharyya
 
Subject Chemistry
 
Description Dynamics of excited state proton transfer(ESPT) in the lysosome region of live lung cells (normaland cancer) is studied by picosecond time-resolved confocal microscopy. For this, we used a fluorescent probe, pyranine (8-
hydroxy-pyrene-1,3,6-trisulfonate, HPTS). From the colocalization of HPTS with a lysotracker dye (lysotracker yellow), we confirmed that HPTS resides in the lysosome for both of the cells. The diffusion coefficient (Dt) in the lysosome region was obtained from fluorescence correlation spectroscopy (FCS). From Dt, the viscosity of lysosome is estimated to be ∼40 and ∼30 cP in the cancer and normal cells, respectively. The rate constants of the elementary steps of ESPT in a normal lung cell (WI38) are compared with those in a lung cancer cell (A549). It is observed that the time constant of the initial proton transfer process in a normal cell (τPT = 40 ps) is similar to that in a cancer cell. The recombination of the geminate ion pair is slightly faster (τrec = 25 ps) in the normal cell than that (τrec = 30 ps) in a cancer cell. The time constant of the dissociation (τdiss) of the geminate ion pair for the cancer cell (τdiss = 80 ps) is 1.5 times
faster compared to that (τdiss = 120 ps) in a normal cell.
 
Publisher American Chemical Society
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2233/1/JOURNAL_OF_PHYSICAL_CHEMISTRY_B__V._119___(_6_)__Special_Issue_SI___Pages_2149%2D2156.pdf
Chowdhury, Rajdeep and Saha, Abhijit and Mandal, Amit Kumar and Jana, Batakrishna and Ghosh, Surajit and Bhattacharyya, Kankan Bhattacharyya (2015) Excited State Proton Transfer in the Lysosome of Live Lung Cells:Normal and Cancer Cells. The Journal of Physical Chemistry B, 119 (6). pp. 2149-2156.
 
Relation http://dx.doi.org/10.1021/jp503804y
http://www.eprints.iicb.res.in/2233/