Record Details

Conversion of Amyloid Fibrils of Cytochrome c to Mature Nanorods through a Honeycomb Morphology

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Conversion of Amyloid Fibrils of Cytochrome c to Mature Nanorods through a Honeycomb Morphology
 
Creator Haldar, Shubhasis
Sil, Pallabi
Thangamuniyandi, Muruganandan
Chattopadhyay, Krishnananda
 
Subject Structural Biology & Bioinformatics
 
Description ABSTRACT: Amyloid species with various morphologies have been found for different proteins and disease systems. In this article, we aim to ask if these morphologies are
unique to a particular protein or if they convert from one to another. Using a heme protein containing iron as the transition-metal activator of aggregation and a negatively
charged surfactant, partial unfolding of the protein and its aggregation have been induced. In the pathway of aggregation, we have observed the formation of several
morphological structures of a single protein, which were visualized directly using atomic force microscopy (AFM). These structures have been found to appear and disappear
with time, and their formation could be monitored under normal buffer conditions and at room temperature without requiring any sophisticated chemical or biological
methodologies. In addition, we have observed the formation of honeycomb-shaped morphology, which may serve as an intermediate. These amyloid-based nanostructures
may have the potential to be explored in therapeutics delivery and other biomedical applications.
 
Publisher American Chemical Society
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2237/1/LANGMUIR__V._31__(_14_)4213%2D4223;2015[29].pdf
Haldar, Shubhasis and Sil, Pallabi and Thangamuniyandi, Muruganandan and Chattopadhyay, Krishnananda (2015) Conversion of Amyloid Fibrils of Cytochrome c to Mature Nanorods through a Honeycomb Morphology. Langmuir, 31 (14). pp. 4212-4223.
 
Relation http://dx.doi.org/10.1021/la5029993
http://www.eprints.iicb.res.in/2237/