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Frequent inactivation of SLIT2 and ROBO1 signaling in head and neck lesions: clinical and prognostic implications

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Title Frequent inactivation of SLIT2 and ROBO1 signaling in head
and neck lesions: clinical and prognostic implications
 
Creator Maiti, Guru Prasad
Ghosh, Amlan
Mondal, Pinaki
Ghosh, Susmita
Chakraborty, Jayanta
Roy, Anup
Roychoudhury, Susanta
Panda, Chinmay Kumar
 
Subject Cancer Biology and Inflammatory Disorder Division
 
Description Objective. The protein SLIT2 and its receptor ROBO1 regulate different cellular processes, such as proliferation, apoptosis,and migration. In this study our aim is to understand the alterations of these genes during development of head and neck squamous cell carcinoma (HNSCC).
Materials and Methods. First, molecular alterations of the genes were analyzed in 30 dysplastic lesions, 128 primary HNSCC samples, and 1 HNSCC cell line. Then alterations were correlated with mRNA expression (n ¼ 22) and protein expression (n ¼29). Finally, the alterations were correlated with different clinicopathologic parameters and clinical outcomes of the patients.
Results. ROBO1 had a comparatively high frequency of deletion (28.5%-54.2%) from dysplastic lesions and subsequent clinical stages than did SLIT2 (16.6-27%). On the contrary, SLIT2 had a high frequency (56.6%-81.2%) of promoter methylation from dysplastic lesions onward compared with ROBO1 (20%-32.8%). Interestingly, alterations of SLIT2 and ROBO1 were high in dysplastic lesions (80%), followed by comparable frequencies (92.5%-95.3%) in subsequent stages of tumor. Alterations of these genes showed concordance with their mRNA/protein expression and significant association with poor patient outcome.
Conclusions. Our data suggest that inactivation of SLIT2 and/or ROBO1 is one of the early events in development of dysplastic lesions of head and neck and has prognostic importance.
 
Publisher Elsevier
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2245/1/ORAL_SURGERY_ORAL_MEDICINE_ORAL_PATHOLOGY_ORAL_RADIOLOGY__V.__119__(_2_)_202%2D212_;2015[71].pdf
Maiti, Guru Prasad and Ghosh, Amlan and Mondal, Pinaki and Ghosh, Susmita and Chakraborty, Jayanta and Roy, Anup and Roychoudhury, Susanta and Panda, Chinmay Kumar (2015) Frequent inactivation of SLIT2 and ROBO1 signaling in head and neck lesions: clinical and prognostic implications. Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontics, 119 (2). pp. 202-212.
 
Relation http://dx.doi.org/10.1016/j.oooo.2014.09.029
http://www.eprints.iicb.res.in/2245/