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PI3K-Mediated Proliferation of Fibroblasts by Calendula officinalis Tincture: Implication in Wound Healing

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Title PI3K-Mediated Proliferation of Fibroblasts by Calendula officinalis Tincture: Implication in Wound Healing
 
Creator Dinda, Manikarna
Dasgupta, Uma
Singh, Namrata
Bhattacharyya2, Debasish
Karmakar, Parimal
 
Subject Structural Biology & Bioinformatics
 
Description Calendula officinalis, a member of theAsteraceae family, is a flowering plant and has been used for its antibacterial,
antifungal, antiviral, antiinflammatory, anticancer and wound healing activity. The mode of action of C. officinalis
tincture on wound healing is poorly understood.Here, we investigated the role of C. officinalis tincture (CDOT) on
cell viability and wound closure. C. officinalis tincture stimulated both proliferation and migration of fibroblasts in a statistically significant manner in a PI3K-dependent pathway. The increase in phosphorylation of FAK(Tyr 397) and
Akt (Ser 473) was detected after treatment of CDOT. Inhibition of the PI3K pathway by wortmannin and LY294002
decreased both cell proliferation and cell migration. HPLC-ESIMS revealed the presence of flavonol glycosides as
themajor compounds of CDOT.Altogether, our results showed that CDOT potentiated wound healing by stimulating
proliferation and migration of fibroblast in a PI3K-dependent pathway, and the identified compounds are likely to be responsible for wound healing activity
 
Publisher John Wiley & Sons
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2250/1/PHYTOTHERAPY_RESEARCH__V._29_(_4_)_607%2D616;2015[31].pdf
Dinda, Manikarna and Dasgupta, Uma and Singh, Namrata and Bhattacharyya2, Debasish and Karmakar, Parimal (2015) PI3K-Mediated Proliferation of Fibroblasts by Calendula officinalis Tincture: Implication in Wound Healing. Phytotherapy Research, 29 (4). pp. 607-616. ISSN 0951-418X
 
Relation http://dx.doi.org/10.1002/ptr.5293
http://www.eprints.iicb.res.in/2250/