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N-glycolylneuraminic acid specific lectin from Pila globosa snail

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Title N-glycolylneuraminic acid specific lectin from Pila globosa snail
 
Creator Swarnakar, Snehasikta
Chowdhury, Partha Sarathi
Sarkar, Manju
 
Subject Cell Biology & Physiology
 
Description A N-glycolylneuraminic acid-specific lectin (PAL) has been
purified from an albumin gland extract of the apple snail, Pila globosa. Purification is conducted on a bovine submaxillary mucin-~$ar~s%-TB affinity matrix followed by gel filtration on a Sepharose 6B column. The lectin agglutinatz; rabbit erythrocytes. The hemagglutination activity is dependent on Ca concentration in a significant manner but with a remark able behaviour. The lectin is a trimeric glycoprotein of native Mr 440 kDa with 25% carbohydrate and is composed of three nonidentical subunits
of molecular weights 190, 145. and 105 kDa. It has a p1 of 7.0. The lectin exhibits a unique and strict specificity toward N-glycolylneuraminic
acid and this phenomenon discriminates it from other known sialic acid binding lectins. The uniqueness indicates the absolute need for a glycolyl substitution on the amino residue and of a glyceryl side chain on the exocyclic part and an axial-COOH group in neuraminic acid. The presence
of an acetyl
 
Publisher Elsevier
 
Date 1991
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2282/1/BIOCHEMICAL_AND_BIOPHYSICAL_RESEARCH_COMMUNICATIONS__V._178_(_1)85%2D94;1991.pdf
Swarnakar, Snehasikta and Chowdhury, Partha Sarathi and Sarkar, Manju (1991) N-glycolylneuraminic acid specific lectin from Pila globosa snail. Biochemical and Biophysical Research Communications, 178 (1). pp. 85-94.
 
Relation http://www.eprints.iicb.res.in/2282/