Record Details

Purification and charactcrisation of a fl-glucosidase (cellobiase)from a mushroom Termitomyces clypeatus

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Purification and charactcrisation of a fl-glucosidase (cellobiase)from a mushroom Termitomyces clypeatus
 
Creator Sengupta, Saswati
Ghosh, Anil K
Sengupta, Subhabrata
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description A ~-ghimsidase with cel~ activity was porifh~d to homogeneity from the culture filtrate of the
Te~tomyces d.rpeatat The enzyme had optimum activity at pH $.0 and temperature 6S°C and was stable up to 60°C
and within pH 2-10. Among the subs/rates tested, p-nitrophenyl.B.D-ghieopyranoside and ¢ellobiose were hydrolysed best by the enzyme. K. and g. values for these substrates were 0.5, 1.25 mM and 95, 91 pmol/min per m~
respectively. The enzyme had low activity towards gentiobiose, salicin and O-metbyl-~glucoside. Glucose and cellebiese
inhibited the fl-D-glucosidase (P~) activity competilivdy with K I of 1.7 and 19 mM, respectively. Molecular
mass of the native enzyme was approximated to be 450 kDa by HPLC, whereas sodium dodecyl sulphate pelyacrylamide
gel electrophoresls indicated a molecular mass of II0 kDa. The high molecular weight enzyme protein was pr--,~mt both
intracellularly and extracellulady from the very early gro~ phase. The enzyme had a pi of 4.5 and appeared to be a
glycoproteln
 
Date 1991
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2286/1/BIOCHIMICA_ET_BIOPHYSICA_ACTA__V._1076__(_2__)_215%2D220;1991.pdf
Sengupta, Saswati and Ghosh, Anil K and Sengupta, Subhabrata (1991) Purification and charactcrisation of a fl-glucosidase (cellobiase)from a mushroom Termitomyces clypeatus. Biachimica et Biophysica Acta.
 
Relation http://www.eprints.iicb.res.in/2286/