Record Details

mRNA Targeting to Endoplasmic Reticulum Precedes Ago Protein Interaction and MicroRNA (miRNA)-mediated Translation Repression in Mammalian Cells

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title mRNA Targeting to Endoplasmic Reticulum Precedes Ago Protein
Interaction and MicroRNA (miRNA)-mediated Translation
Repression in Mammalian Cells
 
Creator Barman, Bahnisikha
Bhattacharyya, Suvendra Nath
 
Subject Molecular & Human Genetics
 
Description MicroRNA (miRNA) binds to the 3�-UTR of its target mRNAs
to repress protein synthesis. Extensive research was done to
understand the mechanism of miRNA-mediated repression in
animal cells. Considering the progress in understanding the
mechanism, information about the subcellular sites of
miRNA-mediated repression is surprisingly limited. In this
study, using an inducible expression system for an miRNA
target message, we have delineated how a targetmRNApasses
through polysome association and Ago2 interaction steps on
rough endoplasmic reticulum (ER) before the miRNA-mediated
repression sets in. From this study, de novo formed target
mRNA localization to the ER-bound polysomes manifested as
the earliest event, which is followed by Ago2 micro-ribonucleoprotein binding, and translation repression of target message. Compartmentalization of this process to rough ER membrane ensures enrichment of miRNA-targeted messages and micro-ribonucleoprotein components on ER upon reaching a steady state
 
Publisher American Society for Biochemistry and Molecular Biology
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2337/1/JOURNAL_OF_BIOLOGICAL_CHEMISTRY__V__290(__41_)_24650%2D24656_;2015;[4].pdf
Barman, Bahnisikha and Bhattacharyya, Suvendra Nath (2015) mRNA Targeting to Endoplasmic Reticulum Precedes Ago Protein Interaction and MicroRNA (miRNA)-mediated Translation Repression in Mammalian Cells. The Journal of Biological Chemistry, 290 (41). pp. 24650-25656.
 
Relation http://dx.doi.org/10.1074/jbc.C115.661868
http://www.eprints.iicb.res.in/2337/