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pH-induced structural change of a multitryptophan protein MPT63 with immunoglobulinlike fold: identification of perturbed tryptophan residue/residues

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Title pH-induced structural change of a multitryptophan protein MPT63 with immunoglobulinlike fold: identification of perturbed tryptophan residue/residues
 
Creator Mukherjee, Manini
Ghosh, Ranendu
Ghosh, Sanjib
Chattopadhyay, Krishnananda
 
Subject Structural Biology & Bioinformatics
 
Description The structural change of M. tuberculosis MPT63, which is predominantly a β-sheet protein having an immunoglobulin
like fold, has been investigated in the pH range 7.5–1.5 using various biophysical techniques along with low-temperature phosphorescence (LTP) spectroscopy. MPT63 contains four Tryptophan (Trp) residues at 26, 48, 82, and 129. Although circular dichroism, steady-state and time-resolved fluorescence, time-resolved anisotropy, 1-aniline-8-naphthalene sulfonic(ANS) acid binding, and analytical ultracentrifuge depict more open largely unfolded structure of MPT63 at pH 1.5 and also more accessible nature of Trp residues to neutral quencher at pH 1.5, it is, however, not possible to assign the specific Trp residue/residues being perturbed. This problem has been resolved using LTP of MPT63, which shows optically resolved four distinct (0, 0) bands corresponding to four Trp residues in the pH range 7.5–3.0. LTP at pH 1.5 clearly reveals that the solvent-exposed Trp 82 and the almost buried Trp 129 are specifically affected compared with Trp 48 and Trp 26. Lys 8 and Lys 27 are predicted to affect Trp 129. Tyrosine residues are found to be silent even at pH 1.5.This type of specific perturbation in a multi-Trp protein has not been addressed before. LTP further indicates that partially
exposed Trp 48 is preferentially quenched by acrylamide compared with other Trp residues at both pH 7.5 and
1.5. The solvent-exposed Trp 82 is surprisingly found to be not quenched by acrylamide at pH 7.5. All the results are
obtained using micromolar concentration of protein and without using any Trp-substituted mutant
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2338/1/JOURNAL_OF_BIOMOLECULAR_STRUCTURE_%26_DYNAMICS__V.__33___(__10_)__2145%2D2160;2015.pdf
Mukherjee, Manini and Ghosh, Ranendu and Ghosh, Sanjib and Chattopadhyay, Krishnananda (2015) pH-induced structural change of a multitryptophan protein MPT63 with immunoglobulinlike fold: identification of perturbed tryptophan residue/residues. Journal of Biomolecular Structure and Dynamics, 33 (10).
 
Relation http://dx.doi.org/10.1080/07391102.2014.992043
http://www.eprints.iicb.res.in/2338/