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Arsenic Exposure through Drinking Water Leads to Senescence and Alteration of Telomere Length in Humans: A Case-Control Study in West Bengal, India

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Title Arsenic Exposure through Drinking Water Leads to
Senescence and Alteration of Telomere Length in
Humans: A Case-Control Study in West Bengal, India
 
Creator Chatterjee, Debmita
Bhattacharjee, Pritha
Sau, Tanmoy Jyoti
Das, Jayanta K
Sarma, Nilendu
Bandyopadhyay, Apurba K
Roy, Sib Sankar
Giri, Ashok K
 
Subject Cell Biology & Physiology
Molecular & Human Genetics
 
Description Arsenic (As) induces pre-malignant and malignant dermatological lesions, non-dermatological health effects and cancers
in humans. Senescence involves telomere length changes and acquisition of senescence-associated secretory phenotype
(SASP), which promotes carcinogenesis. Though in vitro studies have shown that As induces senescence, population based
studies are lacking. We investigated the arsenic-induced senescence, telomere length alteration and its contribution
towards development of As-induced skin cancer. The study participants included 60 each of As-exposed individuals with
skin lesion (WSL), without skin lesions (WOSL) and 60 unexposed controls. Exposure assessment of drinking water and urine
was done. SA b-gal activity, ELISA, and quantification of senescence proteins, alternative lengthening of telomere (ALT)
associated proteins and telomerase activity were performed. Relative telomere length (RTL) was determined by qPCR. A
significantly higher number of senescent cells, over-expression of p53 and p21 were observed in the As-exposed individuals
when compared to unexposed. SASP markers, MMP-1/MMP-3 were significantly higher in the WSL but not IL-6/IL-8. A
significant increase of RTL was observed in the WSL group, which was telomerase-independent but exhibited an overexpression
of ALT associated proteins TRF-1 and TRF-2 with higher increase in TRF-2. An increased risk for developing Asinduced
skin lesions was found for individuals having RTL greater than 0.827 (odds ratio, 13.75; 95% CI: 5.66–33.41;
P
 
Publisher John Wiley & Sons
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2377/1/MOLECULAR_CARCINOGENESIS__Vol_54(__9)__800%2D809;2015.pdf
Chatterjee, Debmita and Bhattacharjee, Pritha and Sau, Tanmoy Jyoti and Das, Jayanta K and Sarma, Nilendu and Bandyopadhyay, Apurba K and Roy, Sib Sankar and Giri, Ashok K (2015) Arsenic Exposure through Drinking Water Leads to Senescence and Alteration of Telomere Length in Humans: A Case-Control Study in West Bengal, India. Molecular Carcinogenesis, 54 (9). pp. 800-809. ISSN 0899-1987
 
Relation http://dx.doi.org/10.1002/mc.22150
http://www.eprints.iicb.res.in/2377/