Record Details

Bioremediation by alkaline protease (AkP)from edible mushroom Termitomyces clypeatus:optimization approach based on statistical design and characterization for diverse applications

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Bioremediation by alkaline protease (AkP)from edible mushroom Termitomyces clypeatus:optimization approach based on statistical design and characterization for diverse
applications
 
Creator Majumder, Rajib
Banik, Samudra Prosad
Ramrakhiania, Lata
Khowala, Suman
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description BACKGROUND: Bioremediation using enzymes has become an attractive approach for removing hazardous chemicals from the
environment. The present study investigated theproduction, optimization andapplications of an extracellular alkalineprotease
(AkP) fromthe edible mushroom Termitomyces clypeatus focusing on bioremediation.
RESULTS: For optimized enzyme production, the variables significantly influencing the protease activitywere screened through
the 11 factorial Plackett–Burman design. The optimum values of the selected variables and their mutual interactions were
determined through response surfacemethodology using the Box–Behnken experimental design. Overall a 10-fold increase in
enzyme activity (1830 UmL-1)was obtained which closely matched the yield predicted by the statisticalmodel (1778.63 UmL-1)
with R2 =0.9819. AkP could efficiently dehair the goat skin, detached bird feather vanes from its shaft and reduce BOD, COD
and pH of tannery waste effluent. The AkP also demonstrated bactericidal property against Staphylococcus aureus as detected
by MIC and flow cytometry analyses.
CONCLUSION: As an ecofriendly alternative, the enzyme showed significant promise for bioremediation and industrial applications
through time-saving bioprocesses. This is first report of alkaline protease from T. clypeatus or from a fungal source with
wide-ranging application potential.
© 2014 Society of Chemical Industry
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2400/1/Majumder_et_al%2D2015%2DJournal_of_Chemical_Technology_and_Biotechnology_(1).pdf
Majumder, Rajib and Banik, Samudra Prosad and Ramrakhiania, Lata and Khowala, Suman (2014) Bioremediation by alkaline protease (AkP)from edible mushroom Termitomyces clypeatus:optimization approach based on statistical design and characterization for diverse applications. Journal of Chemical Technology Biotechnology, 90. pp. 1886-1896.
 
Relation http://dx.doi.org/10.1002/jctb.4500
http://www.eprints.iicb.res.in/2400/