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Transient overexpression ofWerner protein rescues starvation induced autophagy inWerner syndrome cells

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Title Transient overexpression ofWerner protein rescues starvation induced autophagy inWerner syndrome cells
 
Creator Maity, Jyotirindra
Bohr, Vilhelm A
Laskar, Aparna
Karmakar, Parimal
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Reduced autophagy may be associated with normal and pathological aging. Here we report a link between
autophagy and Werner protein (WRNp), mutated in Werner syndrome, the human premature aging Werner
syndrome (WS).WRNmutant fibroblast AG11395 and AG05229 respondweakly to starvation induced autophagy
compared to normal cells. While the fusion of phagosomes with lysosome is normal, WS cells contain fewer
autophagy vacuoles. Cellular starvation autophagy in WS cells is restored after transfection with full length
WRN. Further, siRNA mediated silencing of WRN in the normal fibroblast cell line WI-38 results in decreased
autophagy and altered expression of autophagy related proteins. Thus, our observations suggest that WRN may
have a role in controlling autophagy and hereby cellular maintenance.
 
Publisher Elsevier
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2401/1/Biochimica_et_Biophysica_Acta_1842_(2014)_2387%E2%80%932394.pdf
Maity, Jyotirindra and Bohr, Vilhelm A and Laskar, Aparna and Karmakar, Parimal (2014) Transient overexpression ofWerner protein rescues starvation induced autophagy inWerner syndrome cells. BBA - Biochimica et Biophysica Acta, 1842. pp. 2387-2394.
 
Relation http://dx.doi.org/10.1016/j.bbadis.2014.09.007
http://www.eprints.iicb.res.in/2401/