Record Details

Dual Functionalized Graphene Oxide Serves as a Carrier for Delivering Oligohistidine- and Biotin-Tagged Biomolecules into Cells

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Dual Functionalized Graphene Oxide Serves as a Carrier for Delivering Oligohistidine- and Biotin-Tagged Biomolecules into Cells
 
Creator Jana, Batakrishna
Mondal, Goutam
Biswas, Atanu
Chakraborty, Indrani
Saha, Abhijit
Kurkute, Prashant
Ghosh, Surajit
 
Subject Chemistry
 
Description A versatile method of dual chemical functionalization of graphene oxide (GO) with Tris-[nitrilotris(acetic acid)] (Tris-NTA) and biotin for cellular delivery of oligohistidine- and biotintagged biomolecules is reported. Orthogonally functionalized GO surfaces with Tris-NTA and
biotin to obtain a dual-functionalized GO (DFGO) are prepared and characterized by various spectroscopic and microscopic techniques. Fluorescence microscopic images reveal that DFGO surfaces are capable of binding oligohistidine-tagged biomolecules/proteins and avidin/
biotin-tagged biomolecules/proteins orthogonally. The
DFGO nanoparticles are noncytotoxic in nature and can
deliver oligohistidine- and biotin-tagged biomolecules
simultaneously into the cell
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2413/1/Macromol._Biosci._2013%2C_13%2C_1478%E2%80%931484.pdf
Jana, Batakrishna and Mondal, Goutam and Biswas, Atanu and Chakraborty, Indrani and Saha, Abhijit and Kurkute, Prashant and Ghosh, Surajit (2013) Dual Functionalized Graphene Oxide Serves as a Carrier for Delivering Oligohistidine- and Biotin-Tagged Biomolecules into Cells. Macromolecular Bioscience, 13. pp. 1478-1484.
 
Relation http://www.eprints.iicb.res.in/2413/