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A transient reversal of miRNA-mediated repression controls macrophage activation

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Title A transient reversal of miRNA-mediated repression
controls macrophage activation
 
Creator Mazumdar, Anup
Bose, Mainak
Chakraborty, Abhijit
Chakrabarti, Saikat
Bhattacharyya, Suvendra Nath
 
Subject Molecular & Human Genetics
Structural Biology & Bioinformatics
 
Description In mammalian macrophages, the expression of a number of
cytokines is regulated by miRNAs. Upon macrophage activation, proinflammatory cytokine mRNAs are translated,
although the expression of miRNAs targeting these mRNAs
remains largely unaltered. We show that there is a transient
reversal of miRNA-mediated repression during the early phase of the inflammatory response in macrophages, which leads to the protection of cytokine mRNAs from miRNA-mediated repression. This derepression occurs through Ago2 phosphorylation, which results in its impaired binding to miRNAs and to the corresponding target mRNAs. Macrophages expressing a mutant, non-phosphorylatable AGO2—which remains bound to miRNAs during macrophage activation—have a weakened inflammatory response and fail to prevent parasite invasion. These findings highlight the relevance of the transient relief of miRNA repression for macrophage function
 
Publisher Nature Publishing Group
 
Date 2013
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2417/1/EMBO_REPORTS__Volume__14____Issue__11____Pages__1008%2D1016%2D2013.pdf
Mazumdar, Anup and Bose, Mainak and Chakraborty, Abhijit and Chakrabarti, Saikat and Bhattacharyya, Suvendra Nath (2013) A transient reversal of miRNA-mediated repression controls macrophage activation. EMBO Reports, 14 (11). pp. 1008-1016.
 
Relation http://dx.doi.org/10.1038/embor.2013.149
http://www.eprints.iicb.res.in/2417/