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Selective detection of Escherichia coli DNA using fluorescent carbon spindles

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Title Selective detection of Escherichia coli DNA using
fluorescent carbon spindles
 
Creator Roy, Anurag
Chatterjee, Sabyasachi
Pramanik, Srikrishna
Parukuttyamma , Sujatha Devi
Kumar, Gopinatha Suresh
 
Subject Chemistry
 
Description We investigate the interaction of hydrophilic blue emitting carbon spindles with various deoxyribonucleic acids (DNA) having different base pair compositions, such as Herring testes (HT), calf thymus (CT),Escherichia coli (EC) and Micrococcus lysodeikticus (ML) DNA, to understand the mode of interaction. Interestingly, the fluorescent carbon spindles selectively interacted with E. coli DNA resulting in enhanced fluorescence of the former. Interaction of the same carbon with other DNAs exhibited insignificant changes in fluorescence. In addition, in the presence of EC DNA, the D band in the Raman spectrum attributed to the defect state completely disappeared, resulting in enhanced
crystallinity. Microscopy images confirmed the wrapping of DNA on the carbon spindles leading to the assembly of spindles in the form of flowers. Dissociation of double-stranded DNA occurred upon interaction with carbon spindles, resulting in selective E. coli DNA interaction. The carbon spindles also exhibited a similar fluorescence enhancement upon treating with E. coli bacteria. These
results confirm the possibility of E. coli detection in water and other liquid foods using such fluorescent
carbon
 
Date 2016
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/2533/1/Phys.Chem.Chem.Phys.%2C.pdf
Roy, Anurag and Chatterjee, Sabyasachi and Pramanik, Srikrishna and Parukuttyamma , Sujatha Devi and Kumar, Gopinatha Suresh (2016) Selective detection of Escherichia coli DNA using fluorescent carbon spindles. Phys. Chem. Chem. Phys.. 01p. -08p..
 
Relation http://dx.doi.org/10.1039/c5cp07849k
http://www.eprints.iicb.res.in/2533/